公开(公告)号：US20250161882A1

公开(公告)日：2025-05-22

申请号：US18947506

申请日：2024-11-14

Applicant: ILLUMINA, INC.

Inventor： Yuliia Vyborna ,  Istvan Kocsis ,  Alexandre Richez ,  Davide Garoldini

IPC: B01D67/00 ,  B01L3/00

Abstract: An example of a fluidic device includes a support structure, which defines a well and an interstitial region surrounding the well. An electrode is operatively positioned at a bottom of the well. Membrane fragments are bound over the interstitial region, and a nanopore, a nanopore subunit, or a combination thereof is bound over the interstitial region. The fluidic device may be incorporated into a nanopore instrument.

公开(公告)号：US20240392117A1

公开(公告)日：2024-11-28

申请号：US18653173

申请日：2024-05-02

Applicant: Illumina, Inc.

Inventor： Antonio Conde-Gonzalez ,  Davide Garoldini ,  Yuliia Vyborna ,  Charlotte Vacogne ,  Istvan Kocsis ,  Oliver Uttley ,  Miguel Angel Aleman Garcia ,  Alexandre Richez

IPC: C08L23/06 ,  C08L23/26

Abstract: Methods of making barriers including nanopores and crosslinked amphiphilic molecules, and barriers made using the same, are provided herein. In some examples, a method of forming a barrier between first and second fluids includes forming at least one layer comprising a plurality of amphiphilic molecules, wherein the amphiphilic molecules comprise reactive moieties. The method may include using first crosslinking reactions of the reactive moieties to only partially crosslink amphiphilic molecules of the plurality to one another. The method may include, after using the first crosslinking reactions, inserting the nanopore into the at least one layer. The method may include, after inserting the nanopore, using second crosslinking reactions of the reactive moieties to further crosslink amphiphilic molecules of the plurality to one another.