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1.发明授权公开(公告)号：US08852910B2
公开(公告)日：2014-10-07
申请号：US13856556
申请日：2013-04-04
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Hardold Swerdlow , David James Earnshaw
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
摘要翻译： 本发明涉及修饰的聚合酶，其在糖部分的3'位具有比天然存在的3'羟基更大尺寸的带有取代基的核苷酸类似物的掺入。 还描述了使用聚合酶将核苷酸掺入多核苷酸的方法，特别是在DNA测序的上下文中。
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公开(公告)号：US10017750B2
公开(公告)日：2018-07-10
申请号：US15247561
申请日：2016-08-25
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12 , C12Q1/6869
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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公开(公告)号：US20150024463A1
公开(公告)日：2015-01-22
申请号：US14476387
申请日：2014-09-03
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
摘要翻译： 本发明涉及修饰的聚合酶，其表现出在糖部分的3'位置具有比天然存在的3'羟基更大尺寸的具有取代基的核苷酸类似物的改进掺入。 还描述了使用聚合酶将核苷酸掺入多核苷酸的方法，特别是在DNA测序的上下文中。
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公开(公告)号：US20220025342A1
公开(公告)日：2022-01-27
申请号：US17410647
申请日：2021-08-24
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12 , C12Q1/6874
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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公开(公告)号：US20220010290A1
公开(公告)日：2022-01-13
申请号：US17485004
申请日：2021-09-24
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12 , C12Q1/6869
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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公开(公告)号：US10059928B2
公开(公告)日：2018-08-28
申请号：US15618401
申请日：2017-06-09
发明人： Geoffrey Paul Smith , Roberto Rigatti , Tobias William Barr Ost , Shankar Balasubramanian , Raquel Maria Sanches-Kuiper
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007
摘要： Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
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公开(公告)号：US20160362664A1
公开(公告)日：2016-12-15
申请号：US15247561
申请日：2016-08-25
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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8.发明授权公开(公告)号：US09447389B2
公开(公告)日：2016-09-20
申请号：US14476387
申请日：2014-09-03
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12
CPC分类号： C12N9/1252 , C12Q1/6869 , C12Y207/07007
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
摘要翻译： 本发明涉及修饰的聚合酶，其在糖部分的3'位具有比天然存在的3'羟基更大尺寸的带有取代基的核苷酸类似物的掺入。 还描述了使用聚合酶将核苷酸掺入多核苷酸的方法，特别是在DNA测序的上下文中。
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公开(公告)号：US09273352B2
公开(公告)日：2016-03-01
申请号：US14137434
申请日：2013-12-20
发明人： Geoffrey Paul Smith , Roberto Rigatti , Tobias William Barr Ost , Shankar Balasubramanian , Raquel Maria Sanches-Kuiper
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007
摘要： Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
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公开(公告)号：US11473067B2
公开(公告)日：2022-10-18
申请号：US17410647
申请日：2021-08-24
发明人： Geoffrey Paul Smith , David Mark Dunstan Bailey , Raquel Maria Sanches-Kuiper , Harold Swerdlow , David James Earnshaw
IPC分类号： C12N9/12 , C12Q1/6869
摘要： The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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