公开(公告)号：US10184025B2

公开(公告)日：2019-01-22

申请号：US14161773

申请日：2014-01-23

Applicant: IMRA AMERICA, INC.

Inventor： Yuki Ichikawa ,  Andrius Marcinkevicus ,  Masayuki Ito ,  Wei Qian

IPC: B01J13/00 ,  C08G65/328 ,  B82Y30/00

Abstract: The present disclosure is directed to methods of preparing stable suspensions of precious metal nanoparticles and methods for attaching bio-molecules to the nanoparticles. The formation of nanoparticles can be accomplished by either chemical synthesis or pulsed laser ablation in a liquid. The present disclosure reveals the importance of controlling the conductivity of the dispersion medium during pulsed laser ablation in a liquid to control the particle size of the nanoparticles. The present disclosure also reveals the importance of adjusting and maintaining the conductivity in a range of 25 μS/cm or less during storage of the nanoparticles and just prior to performing bioconjugation reactions. The control of conductivity is an important process for maintaining the nanoparticles as a stable non-aggregated colloidal suspension in a dispersion medium.