公开(公告)号：US10092661B2

公开(公告)日：2018-10-09

申请号：US14187416

申请日：2014-02-24

Applicant: IMRA America, Inc.

Inventor： Wei Qian ,  Makoto Murakami ,  Yuki Ichikawa ,  Yong Che

IPC: A61K49/00 ,  G01N21/64 ,  G01N33/531 ,  G01N33/543 ,  A61K9/00 ,  A61K9/16 ,  B01J13/00 ,  A61K41/00 ,  A61K47/10 ,  G01N21/65 ,  B82Y5/00 ,  B82Y40/00 ,  G01N21/35 ,  B82Y30/00

Abstract: In the present invention, a method of producing stable bare colloidal gold nanoparticles is disclosed. The nanoparticles can subsequently be subjected to partial or full surface modification. The method comprises preparation of colloidal gold nanoparticles in a liquid by employing a top-down nanofabrication method using bulk gold as a source material. The surface modification of these nanoparticles is carried out by adding one or multiple types of ligands each containing functional groups which exhibit affinity for gold nanoparticle surfaces to produce the conjugates. Because of the high efficiency and excellent stability of the nanoparticles produced by this method, the fabricated gold nanoparticle conjugates can have surface coverage with functional ligands which can be tuned to be any percent value between 0 and 100%.

公开(公告)号：US20150110882A1

公开(公告)日：2015-04-23

申请号：US14057609

申请日：2013-10-18

Applicant: JAPANESE FOUNDATION FOR CANCER RESEARCH ,  IMRA America, Inc.

Inventor： Yuki Ichikawa ,  Kiyotaka Shiba

IPC: A61K9/14 ,  G01N33/58 ,  A61K47/10 ,  G01N33/574 ,  A61K38/10 ,  A61K47/02

CPC classification number: G01N33/54346 ,  A61K9/14 ,  A61K38/10 ,  A61K47/02 ,  A61K47/10 ,  A61K49/0004 ,  G01N33/553 ,  G01N33/574 ,  G01N33/5748 ,  G01N33/57492 ,  G01N33/587 ,  G01N2333/705 ,  G01N2333/82

Abstract: It is intended to provide a stable metallic nanostructure that causes no aggregation when surface-modified with biomolecule-reactive functional molecules. 30 to 90% of the surface of a metallic nanostructure is covered with at least one or more types of colloid-stabilizing functional molecules. The remaining portions on the surface of the metallic nanostructure are further covered with one or more types of biologically functional molecules.

Abstract translation: 旨在提供稳定的金属纳米结构，当用生物分子反应性功能分子进行表面改性时，其不会引起聚集。 金属纳米结构表面的30〜90％被至少一种以上胶体稳定的功能分子覆盖。 金属纳米结构表面上的剩余部分进一步用一种或多种生物功能分子覆盖。

公开(公告)号：US20130327987A1

公开(公告)日：2013-12-12

申请号：US13968464

申请日：2013-08-16

Applicant: IMRA America, Inc.

Inventor： Yuki Ichikawa ,  Zhendong HU ,  Bing LU ,  Yong Che

IPC: C09K11/02

CPC classification number: C09K11/025 ,  C09K11/08 ,  Y10T428/2982

Abstract: A method of forming nanometer sized fine particles of functional ceramic from a bulk functional ceramic, particularly fine particles of phosphorous ceramics from a bulk phosphor material is disclosed. The method relies on irradiation of a bulk phosphorous ceramic in a liquid with an ultrashort-pulsed-laser-fragmentation beam to thereby form nanometer sized particles of the phosphorous ceramic. The method is unique in that the generated particles retain the chemical and crystalline properties of the bulk phosphorous ceramic. The generated solutions are stable colloids from which the particles can be isolated or used as is.

Abstract translation: 公开了一种从体积功能陶瓷形成纳米尺寸的功能性陶瓷微粒的方法，特别是来自块状磷光体材料的磷陶瓷的细颗粒的方法。 该方法依赖于用超短脉冲激光破碎光束在液体中照射大块磷陶瓷，从而形成纳米尺寸的磷陶瓷颗粒。 该方法的独特之处在于所产生的颗粒保留了大块磷陶瓷的化学和结晶性质。 产生的溶液是稳定的胶体，颗粒可从其中分离或原样使用。

公开(公告)号：US20220239053A1

公开(公告)日：2022-07-28

申请号：US17537885

申请日：2021-11-30

Applicant: IMRA America, Inc.

Inventor： Hiroyuki Yada ,  Yuki Ichikawa

IPC: H01S3/0941 ,  H01S3/16 ,  H01S3/094 ,  H01S3/23

Abstract: A laser amplifier for a green laser pulse includes at least one gain medium doped with praseodymium and at least one gallium nitride based diode laser for pumping the gain medium. A green seed laser pulse going through the gain medium becomes an amplified green laser pulse.

公开(公告)号：US20190324023A1

公开(公告)日：2019-10-24

申请号：US15958241

申请日：2018-04-20

Applicant: IMRA America, Inc. ,  JAPANESE FOUNDATION FOR CANCER RESEARCH

Inventor： Yuki Ichikawa ,  Kiyotaka Shiba ,  Sachiko Matsumura

IPC: G01N33/543

Abstract: The present disclosure provides examples of methods and kits for easily detecting, classifying and/or purifying extracellular vesicles. The method can include subjecting, to a density gradient centrifugation, a sample solution in which the extracellular vesicles and nanoparticles coated with ligand that specifically binds to molecule present on the surface of the extracellular vesicles are mixed.

公开(公告)号：US10184025B2

公开(公告)日：2019-01-22

申请号：US14161773

申请日：2014-01-23

Applicant: IMRA AMERICA, INC.

Inventor： Yuki Ichikawa ,  Andrius Marcinkevicus ,  Masayuki Ito ,  Wei Qian

IPC: B01J13/00 ,  C08G65/328 ,  B82Y30/00

Abstract: The present disclosure is directed to methods of preparing stable suspensions of precious metal nanoparticles and methods for attaching bio-molecules to the nanoparticles. The formation of nanoparticles can be accomplished by either chemical synthesis or pulsed laser ablation in a liquid. The present disclosure reveals the importance of controlling the conductivity of the dispersion medium during pulsed laser ablation in a liquid to control the particle size of the nanoparticles. The present disclosure also reveals the importance of adjusting and maintaining the conductivity in a range of 25 μS/cm or less during storage of the nanoparticles and just prior to performing bioconjugation reactions. The control of conductivity is an important process for maintaining the nanoparticles as a stable non-aggregated colloidal suspension in a dispersion medium.

公开(公告)号：US09999865B2

公开(公告)日：2018-06-19

申请号：US14312824

申请日：2014-06-24

Applicant: IMRA America, Inc.

Inventor： Yuki Ichikawa ,  Andrius Marcinkevicus

IPC: B01J13/00 ,  B82Y40/00

CPC classification number: B01J13/0043 ,  B82Y40/00

Abstract: Disclosed is a method for making a colloidal suspension of precious metal nanoparticles. The method comprises providing a target material comprising a precious metal in a liquid dispersion medium in an ablation container. The dispersion medium has an electrical conductivity within a predetermined conductivity range. Laser pulses are used to generate the nanoparticles from the target in the container. While generating the nanoparticles the electrical conductivity of the dispersion medium is monitored and maintained within the predetermined range and thereby the generated nanoparticles are produced within a predetermined size range. The generated nanoparticles are used to form a colloidal suspension.