公开(公告)号：US20150027953A1

公开(公告)日：2015-01-29

申请号：US14371193

申请日：2013-01-09

Applicant: INDIANA UNIVERSITY RESEARCH AND TECHNOLOGY CORPORATION

Inventor： Amanda K. Peterson Mann ,  Benjamin F. Mann ,  Sara E. Skrabalak ,  Milos V. Novotny

IPC: B01J20/286 ,  B01D15/38 ,  B01J20/283 ,  B01D15/08

CPC classification number: B01J20/286 ,  B01D15/08 ,  B01D15/3804 ,  B01D15/3823 ,  B01J20/103 ,  B01J20/28019 ,  B01J20/28061 ,  B01J20/28085 ,  B01J20/28092 ,  B01J20/283 ,  B01J20/3057 ,  B01J20/3078 ,  B01J20/3092 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3274 ,  B01J2220/80 ,  C01B33/18 ,  C07K1/22

Abstract: The present invention discloses a silica particle having a diameter less than or equal to 2 μη, wherein the particle is spherical and comprises interconnected pores having a diameter in the range from 50 nm to 300 nm. The silica particle is preferably produced by spray pyrolysis (=spray drying) of a silica colloid. In the production process, porosity is introduced by means of an inorganic salt, such as NaCl, KCI, LiCl, NaNO3 or Ll NO3, which serves as a pore template. The silica particle may further be functionalized with proteins, peptides, nucleic acids, polysaccharides and proteoglycans, preferably concanavalin A or avidin. The present invention further discloses the use of the silica particle in chromatography, in particular in affinity chromatography.

Abstract translation: 本发明公开了直径小于或等于2μe的二氧化硅颗粒，其中颗粒是球形的并且包含直径在50nm至300nm范围内的互连孔。 二氧化硅颗粒优选通过二氧化硅胶体的喷雾热解（=喷雾干燥）来制备。 在生产过程中，通过无机盐，例如NaCl，KCl，LiCl，NaNO 3或者作为孔模板的L 1 NO 3引入孔隙率。 二氧化硅颗粒还可以用蛋白质，肽，核酸，多糖和蛋白聚糖功能化，优选是伴刀豆球蛋白A或抗生物素蛋白。 本发明进一步公开了二氧化硅颗粒在色谱中的用途，特别是在亲和层析中。