公开(公告)号：US11753636B2

公开(公告)日：2023-09-12

申请号：US16756851

申请日：2018-10-18

申请人： INFUSION TECH

发明人： Yong Shin ,  Choong Eun Jin

IPC分类号： C12N15/10 ,  G01N1/40

CPC分类号： C12N15/1006 ,  G01N1/4044

摘要： The present invention relates to a method of enriching a pathogen using an homobifunctional imidoester group, and the method of enriching a pathogen and extracting nucleic acids using homobifunctional imidoester compound (DMA, DMP, DMS) according to the present invention can quickly extract a small amount of pathogen contained in a sample without the use of special equipment and can be used as in situ diagnosis, and since it is possible to enrich a pathogen and extract nucleic acids simultaneously in one tube or a chip, it has the advantage of being more efficient than the conventional method, saving time and cost, and being easy to use.

公开(公告)号：US11603526B2

公开(公告)日：2023-03-14

申请号：US16759366

申请日：2018-10-18

申请人： INFUSION TECH

发明人： Yong Shin ,  Huifang Liu

IPC分类号： C12N15/10 ,  C12Q1/6806

摘要： The present invention relates to a method for lysing pathogen lysis and a method for extracting nucleic acid using zinc oxide nanostar, and the method for extracting nucleic acid using zinc oxide nanostar according to the present invention can extract nucleic acids by lysing cells of a pathogen without using a lysis buffer and can extract nucleic acid of high-purity and high-concentration by preventing nucleic acid degradation and fragmentation through various substances including salts contained in the lysis buffer at high concentration. In addition, the zinc oxide nanostar of the present invention (200 to 900 nm) has superior cell lysis capacity compared to the conventional zinc oxide nanoparticles (20 to 50 nm), thereby increasing the nucleic acid extraction efficiency and can extract at room temperature without a heating step to use as a field diagnostic method.

公开(公告)号：US11584924B2

公开(公告)日：2023-02-21

申请号：US16326376

申请日：2017-08-24

申请人： INFUSION TECH

发明人： Yong Shin

IPC分类号： C12N15/10 ,  B01L3/00 ,  C12N1/02 ,  C12Q1/24

摘要： A method for concentrating microorganisms, includes modifying an object by introducing an amine group into the object (step 1); and contacting a sample including a microorganism and dimethyl 3,3′-dithiobispropionimidate (DTBP) each other on the modified object (step 2), wherein the object is any one of a thin film device, a magnetic bead, a ring resonator, and a nanoparticle.

公开(公告)号：US10907146B2

公开(公告)日：2021-02-02

申请号：US16302093

申请日：2017-05-15

申请人： INFUSION TECH

发明人： Yong Shin

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  B01L3/00

摘要： The present invention relates to a ribonucleic acid (RNA) extraction method using a solid subject, the method including activating a subject with a reactive amine group, injecting a sample having 1×101 to 1×103 cells/ml and a dimethyl suberimidate (DMS) compound or a dimethyl pimelimidate (DMP) compound into the subject, and forming a complex having the RNA within the sample and the compound, and extracting the RNA by treating elution buffer to the subject on which the complex is formed. The subject, particularly, the thin film device used for extracting the RNA, has improved hydrophilicity compared to the conventional silicon substrate, so that RNA is extracted more efficiently.