公开(公告)号：US12221596B2

公开(公告)日：2025-02-11

申请号：US17268383

申请日：2020-12-04

Applicant: INJE UNIVERSITY INDUSTRY—ACADEMIC COOPERATION FOUNDATION

Inventor： Se Hoon Jeong ,  Sung Tae Kim

IPC: C12N5/077 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  C12M3/06 ,  C12N5/071

Abstract: A method for manufacturing a skin chip according to an exemplary embodiment of the present disclosure may include: a step of forming first and second PDMS layers disposed on both surfaces of a porous membrane and each having a microfluidic channel through which a culture medium is transferred to both surfaces of the porous membrane; a step of forming first and second MEA substrate layers disposed on the outer surfaces of the first and second PDMS layers, respectively, and having metal electrodes for measurement of TEER arranged at positions corresponding to the channels; and a step of forming first and second PMMA layers disposed on the outer surfaces of the first and second MEA substrate layers, respectively. In the method for manufacturing a skin chip according to in an exemplary embodiment of the present disclosure, the porous membrane may be made of a polycarbonate having pores of a predetermined size.