利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.056 秒)

    METHOD FOR SCREENING A SUBJECT FOR A CANCER
    1.
    发明申请
    METHOD FOR SCREENING A SUBJECT FOR A CANCER 有权

    公开(公告)号：US20220290244A1

    公开(公告)日：2022-09-15

    申请号：US17631908

    申请日：2020-07-31

    申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Institut Régional du Cancer de Montpellier ,  Université de Montpellier

    发明人： Alain THIERRY

    IPC分类号： C12Q1/6886

    摘要： The present invention relates to the field of cancer diagnostic. Here, the inventors observed by using conventional DSP sequencing method that size profile of double stranded DNA fragments obtained from cell free nucleic acids (cfDNA) may discriminate cfDNA from healthy and cancer derived subjects as previously observed (Jiang et al). Contrary to the prior art, the inventors determined specific double stranded DNA fragments or range of fragments and showed that these the number of these specific fragments are different between healthy and cancerous subjects. The number of double stranded DNA fragments as quantified from CfDNA are rather lower or higher when derived from healthy subject than from cancer subject. The invention provides description of calculation or biomarker from the identification of specific DNA fragments, specific ratios for different size or range of double stranded DNA fragment towards discriminating cancer to healthy plasma. The present invention relates to a method for screening a subject for a cancer by determining the level of double stranded DNA fragments in a sample.

    METHOD FOR SCREENING A SUBJECT FOR CANCER
    2.
    发明申请
    METHOD FOR SCREENING A SUBJECT FOR CANCER 有权

    公开(公告)号：US20210172019A1

    公开(公告)日：2021-06-10

    申请号：US16770351

    申请日：2018-12-06

    申请人： INSERM (Institut National de la Santé et de la Recherche Médicale) ,  Université de Montpellier ,  Institut Régional du Cancer de Montpellier

    发明人： Alain THIERRY ,  Cynthia SANCHEZ

    IPC分类号： C12Q1/6886 ,  C12Q1/6806

    摘要： The present invention relates to the diagnostic of cancerous subject. Indeed, the inventors observed by using a Q-PCR based methods and sequencing methods that quantification of specific single stranded DNA fragments obtained from cell free nucleic acids (cfDNA) may discriminate cfDNA from healthy and cancer derived subjects. Single stranded DNA fragments obtained from CfDNA or specific range of single stranded DNA fragments are rather lower or higher when derived from healthy subject than from cancer subject. More, specific ratios for different size or range of single stranded DNA fragments varies between cancer subjects and healthy individuals. These values are sufficiently and significantly different to be used as values to determine whether a human subject may have cancer or not as a screening test. Thus, the invention relates to a method for screening a subject for a cancer comprising the steps of extracting and denaturing cfDNA, determining the single strand fragment level upon their size distribution, and calculate these former values to screen an individual for cancer.

    METHODS FOR SCREENING A SUBJECT FOR A CANCER
    4.
    发明申请
    METHODS FOR SCREENING A SUBJECT FOR A CANCER 审中-公开

    公开(公告)号：US20170240975A1

    公开(公告)日：2017-08-24

    申请号：US15518392

    申请日：2015-10-20

    申请人： INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE ,  INSTITUT REGIONAL DU CANCER DE MONTPELLIER ,  UNIVERSITE DE MONTPELLIER

    发明人： Alain THIERRY ,  Safia EL MESSAOUDI

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/118 ,  C12Q2600/158

    摘要： The present invention relates to methods for screening a subject for a cancer. In particular, the present invention relates to a method (A) for screening a subject for a cancer comprising the steps of i) extracting the cell free nucleic acids from a sample obtained from the subject, ii) determining the total concentration of mitochondrial cell free nucleic acids, ii) determining the total concentration of nuclear cell free nucleic acids iv) calculating the ratio of the level determined at step ii) to the concentration determined at step iii), v) comparing ratio determined at step iv) with a predetermined corresponding reference value and vi) concluding that the subject suffers from a cancer when the ratio determined at step iv) is lower than the predetermined corresponding reference value or concluding that the subject does not suffer from a cancer when the ratio determined at step iv) is higher than the predetermined corresponding reference value.

    METHOD FOR SCREENING A SUBJECT FOR CANCER
    7.
    发明公开
    METHOD FOR SCREENING A SUBJECT FOR CANCER 审中-公开

    公开(公告)号：US20230399702A1

    公开(公告)日：2023-12-14

    申请号：US18355853

    申请日：2023-07-20

    申请人： INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECERCHE MÉDICALE ,  UNIVERSITÉ DE MONTPELLIER ,  INSTITUT RÉGIONAL DU CANCER DE MONTPELLIER

    发明人： Alain THIERRY ,  Cynthia SANCHEZ

    IPC分类号： C12Q1/6886 ,  C12Q1/6806

    CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/112

    摘要： The present invention relates to the diagnostic of cancerous subject. Indeed, the inventors observed by using a Q-PCR based methods and sequencing methods that quantification of specific single stranded DNA fragments obtained from cell free nucleic acids (cfDNA) may discriminate cfDNA from healthy and cancer derived subjects. Single stranded DNA fragments obtained from CfDNA or specific range of single stranded DNA fragments are rather lower or higher when derived from healthy subject than from cancer subject. More, specific ratios for different size or range of single stranded DNA fragments varies between cancer subjects and healthy individuals. These values are sufficiently and significantly different to be used as values to determine whether a human subject may have cancer or not as a screening test. Thus, the invention relates to a method for screening a subject for a cancer comprising the steps of extracting and denaturing cfDNA, determining the single strand fragment level upon their size distribution, and calculate these former values to screen an individual for cancer.