公开(公告)号：US3714445A

公开(公告)日：1973-01-30

申请号：US3714445D

申请日：1970-11-09

申请人： INST NAT RECH AGRENOMIQUE ,  GOURDON ETS

发明人： BLACHERE H ,  JAMART G

IPC分类号： B01L3/02 ,  G01N1/14 ,  G01N21/15 ,  G01N21/27 ,  G01N21/26

CPC分类号： C12M41/36 ,  B01L3/0227 ,  G01N1/14 ,  G01N21/15 ,  G01N21/27 ,  Y10S435/808

摘要： A combined sampling and measuring apparatus for optical density measurements of the growth of microbial cultures in large capacity vessel, said apparatus comprising a cylindrical vertical transparent tube mounted in shunt with a closed circuit tubular system in which circulates a fraction of the microbial culture from the vessel, a piston able to slide in the transparent tube, a toric seal on the piston performing the additional function of cleaning the inner wall of the transparent tube when the piston is moved up and down in the transparent tube, a light source and a photo-cell in diametrical opposition; the piston has a diametrical port from side to side to allow the light beam from the source to get the photo-cell when the piston is between the source and the cell, and the piston is provided with means in order that the axis of the port be maintained parallel to the path of the light rays. The whole is completed by a control block performing a succession of operations including: cleaning of the transparent tube, optical calibration, filling of the tube, and optical density measurement of the culture.

摘要翻译： 用于在大容量容器中微生物培养物的生长的光密度测量的组合采样和测量装置，所述装置包括圆柱形垂直透明管，其安装成与闭路管状系统分流，其中将一部分微生物培养物从容器中循环 能够在透明管中滑动的活塞，当活塞在透明管中上下移动时，活塞上的复曲面密封件执行清洁透明管的内壁的附加功能，光源和照相机， 直接反对的细胞 活塞具有从一侧到另一侧的直径端口，以允许来自源的光束在活塞位于源和电池之间时获得光电池，并且活塞设有装置，以便端口的轴线 保持平行于光线的路径。