专利检索 ap:("INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE" OR "NANTES UNIVERSITÉ" OR "HCS PHARMA" OR "CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE)" OR "CHU DE NANTES") AND inv:"Zied SOUGUIR" 第 1 页

1.

发明公开
LIVER ORGANOID MANUFACTURING METHODS, LIVER ORGANOIDS OBTAINED WITH THE SAME, AND USES THEREOF 审中-公开

公开(公告)号：US20240279612A1

公开(公告)日：2024-08-22

申请号：US18553316

申请日：2022-03-31

申请人： INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE , NANTES UNIVERSITÉ , HCS PHARMA , CNRS (CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE) , CHU DE NANTES

发明人： Karim SI-TAYEB , Amandine CAILLAUD , Nathalie MAUBON , Zied SOUGUIR , Meryl ROUDAUT

IPC分类号： C12N5/071

CPC分类号： C12N5/0671 , C12N2500/02 , C12N2501/115 , C12N2501/12 , C12N2501/15 , C12N2501/155 , C12N2501/2306 , C12N2501/727 , C12N2501/999 , C12N2506/45 , C12N2513/00 , C12N2533/54 , C12N2537/10

摘要： The present disclosure relates to methods for preparing a liver organoid, as well as liver organoids obtained with the same and uses thereof. It was observed that, in presence of a set of suitable cell culture media, a use of a 3D porous scaffold and of alternated steps of hypoxic and normoxic conditions was able to drive the differentiation of stems cells in functional liver organoids comprising at least in hepatocytes, cholangiocytes, stellate cells, and sinusoidal endothelial cells. The obtained liver organoids expressed liver-specific genes, responded to LDL internalization, showed lipid accumulation and presented a strong activity of CYP enzymes.