公开(公告)号：US20160177324A1

公开(公告)日：2016-06-23

申请号：US14787408

申请日：2013-04-28

Applicant: INSTITUTE OF BOTANY, THE CHINESE ACADEMY OF SCIENCES

Inventor： Liangsheng WANG ,  Hui DU ,  Qingyan SHU

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/825 ,  C12N9/1007 ,  C12N15/8205 ,  C12Y201/01

Abstract: The present invention provides novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. The said protein having an amino acid sequence shown in SEQ ID NO: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence. The said gene comprising the nucleotide sequence shown in SEQ ID NO: 1, or a gene which hybridizes with said gene under stringent conditions and encodes a protein, which has anthocyanin 3′-O-methyltransferase or 3′,5′-O-methyltransferase activity. The present invention also provides a method for obtaining the transgenic plant used the above-mentioned gene.

Abstract translation: 本发明提供与类黄酮O-甲基转移酶（FOMT）相关的新型蛋白质和基因及其用途。 所述蛋白质具有SEQ ID NO：3所示的氨基酸序列，或在所述氨基酸序列中具有一个或多个氨基酸的缺失，取代或插入的氨基酸序列。 所述基因包含SEQ ID NO：1所示的核苷酸序列，或在严格条件下与所述基因杂交并编码具有花青素3'-O-甲基转移酶或3'，5'-O-甲基转移酶 活动。 本发明还提供了获得使用上述基因的转基因植物的方法。

公开(公告)号：US20160319290A2

公开(公告)日：2016-11-03

申请号：US14787408

申请日：2013-04-28

Applicant: INSTITUTE OF BOTANY, THE CHINESE ACADEMY OF SCIENCES

Inventor： Liangsheng WANG ,  Hui DU ,  Qingyan SHU

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/825 ,  C12N9/1007 ,  C12N15/8205 ,  C12Y201/01

Abstract: The present invention provides novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. The said protein having an amino acid sequence shown in SEQ ID NO: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence. The said gene comprising the nucleotide sequence shown in SEQ ID NO: 1, or a gene which hybridizes with said gene under stringent conditions and encodes a protein, which has anthocyanin 3′—O-methyltransferase or 3′,5′—O-methyltrasnferase activity. The present invention also provides a method for obtaining the transgenic plant used the above-mentioned gene.

Abstract translation: 本发明提供与类黄酮O-甲基转移酶（FOMT）相关的新型蛋白质和基因及其用途。 所述蛋白质具有SEQ ID NO：3所示的氨基酸序列，或在所述氨基酸序列中具有一个或多个氨基酸的缺失，取代或插入的氨基酸序列。 所述基因包含SEQ ID NO：1所示的核苷酸序列，或在严格条件下与所述基因杂交并编码具有花青素3'-O-甲基转移酶或3'，5'-O-甲基酪氨酸激酶的蛋白质的基因 活动。 本发明还提供了获得使用上述基因的转基因植物的方法。