专利检索 ap:("INTEGRATED DNA TECHNOLOGIES, INC.") AND inv:"Mollie Schubert" 第 1 页

1.

发明公开
USE OF UNIQUE MOLECULAR IDENTIFIERS FOR IMPROVED ACCURACY OF LONG READ SEQUENCING AND CHARACTERIZATION OF CRISPR EDITING 审中-公开

公开(公告)号：US20230366020A1

公开(公告)日：2023-11-16

申请号：US18316454

申请日：2023-05-12

申请人： INTEGRATED DNA TECHNOLOGIES, INC.

发明人： Mollie Schubert , Thomas Osborne , Matthew McNeill , Garrett Rettig

IPC分类号： C12Q1/6869 , C12Q1/6851

CPC分类号： C12Q1/6869 , C12Q1/6851 , C12Q1/6806

摘要： Described herein is a system and process for long read sequencing using PCR primers with incorporated Unique Molecular Identifiers (UMIs) for generating a single molecule consensus for each starting molecule in the sample population. This method reduces the sequencing error rate by generating a consensus from the individual reads in each UMI group, averaging out sequencing errors to give better confidence in the actual sequence, to allow for increased accuracy of quantifying the precise knock-in event, and reporting perfect HDR integration.