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    Method for Making Acylated Polypeptides
    1.
    发明申请
    Method for Making Acylated Polypeptides 有权
    制备酰化多肽的方法

    公开(公告)号：US20100055738A1

    公开(公告)日：2010-03-04

    申请号：US12543027

    申请日：2009-08-18

    申请人： Ib Jonassen ,  Michi Egel- Mitani ,  Per Balschmidt ,  Jan Markussen ,  Ivan Diers ,  Thomas Borglum Kjeldsen

    发明人： Ib Jonassen ,  Michi Egel- Mitani ,  Per Balschmidt ,  Jan Markussen ,  Ivan Diers ,  Thomas Borglum Kjeldsen

    IPC分类号： C12P21/06 ,  C07K4/00

    CPC分类号： C07K1/006 ,  C07K1/1077

    摘要： The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ε-amino groups.

    摘要翻译： 本发明涉及通过在随后的体外步骤中表达待酰化的所需多肽的前体分子，在转化的宿主细胞中产生多肽的方法。 本发明还涉及用于所要求保护的方法的DNA序列，载体和转化的宿主细胞。 此外，本发明涉及所需多肽的某些前体和某些酰化方法。 本发明提供了在某些赖氨酸和氨基中优先酰化多肽的方法。

    Method for making acylated polypeptides
    2.
    发明授权
    Method for making acylated polypeptides 有权
    制备酰化多肽的方法

    公开(公告)号：US08835132B2

    公开(公告)日：2014-09-16

    申请号：US12543027

    申请日：2009-08-18

    申请人： Ib Jonassen ,  Michi Egel-Mitani ,  Per Balschmidt ,  Jan Markussen ,  Ivan Diers ,  Thomas Borglum Kjeldsen

    发明人： Ib Jonassen ,  Michi Egel-Mitani ,  Per Balschmidt ,  Jan Markussen ,  Ivan Diers ,  Thomas Borglum Kjeldsen

    IPC分类号： C07K1/00 ,  C07K1/107

    CPC分类号： C07K1/006 ,  C07K1/1077

    摘要： The present invention related to a method of producing polypeptides in transformed host cells by expressing a precursor molecule of the desired polypeptide which are to be acylated in a subsequent in vitro step. The invention is also related to DNA-sequences, vectors and transformed host cells for use in the claimed method. Further, the present invention is related to certain precursors of the desired polypeptides and certain acylation methods. The invention provides a method for making polypeptides being preferentially acylated in certain lysine ε-amino groups.

    摘要翻译： 本发明涉及通过在随后的体外步骤中表达待酰化的所需多肽的前体分子，在转化的宿主细胞中产生多肽的方法。 本发明还涉及用于所要求保护的方法的DNA序列，载体和转化的宿主细胞。 此外，本发明涉及所需多肽的某些前体和某些酰化方法。 本发明提供了在某些赖氨酸和氨基中优先酰化多肽的方法。

    Acylated insulin
    8.
    发明申请
    Acylated insulin 审中-公开
    酰化胰岛素

    公开(公告)号：US20060030518A1

    公开(公告)日：2006-02-09

    申请号：US11169100

    申请日：2005-06-28

    申请人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Andersen ,  Jan Markussen

    发明人： Svend Havelund ,  John Halstrom ,  Ib Jonassen ,  Asser Andersen ,  Jan Markussen

    IPC分类号： A61K38/28 ,  C07K14/62

    CPC分类号： C07K14/62 ,  A61K38/00 ,  Y10S514/866

    摘要： The present invention relates to protracted human insulin derivatives in which the A21 and the B3 amino acid residues are, independently, any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys; PheB1 may be deleted; the B30 amino acid residue is (a) a non-codable, lipophilic amino acid having from 10 to 24 carbon atoms, in which case an acyl group of a carboxylic acid with up to 5 carbon atoms is bound to the ε-amino group of LysB29; or (b) the B30 amino acid residue is deleted or is any amino acid residue which can be coded for by the genetic code except Lys, Arg and Cys, in any of which cases the C-amino group of LysB29 has a lipophilic substituent; and any Zn2+ complexes thereof with the proviso that when B30 is Thr or Ala and A21 and B3 are both Asn, and PheB1 is present, then the insulin derivative is always present as a Zn2+ complex.

    摘要翻译： 本发明涉及延长的人胰岛素衍生物，其中A21和B3氨基酸残基独立地是可由遗传密码编码的除Lys，Arg和Cys之外的任何氨基酸残基; Phe 可以被删除; B30氨基酸残基是（a）具有10-24个碳原子的不可编码的亲脂性氨基酸，在这种情况下，具有至多5个碳原子的羧酸的酰基与ε-氨基的ε-氨基结合 B29 或（b）B30氨基酸残基被缺失，或者是任何氨基酸残基，其可以通过除Lys，Arg和Cys之外的遗传密码进行编码，在任何情况下，Lys B29的C-氨基， / SUP>具有亲油取代基; 和其任何Zn 2+ 2 +配合物，条件是当B30是Thr或Ala，A21和B3都是Asn和Phe B1时，则胰岛素衍生物是 总是作为Zn 2+复合物存在。