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公开(公告)号:US20070059689A1
公开(公告)日:2007-03-15
申请号:US10496751
申请日:2002-11-29
申请人: Ignacio Aguirrezabalaga , Nerea Allende , Alfredo Brana , Carmen Mendez , Leticia Rodriquez , Jose Salas
发明人: Ignacio Aguirrezabalaga , Nerea Allende , Alfredo Brana , Carmen Mendez , Leticia Rodriquez , Jose Salas
IPC分类号: C12Q1/68 , C07H21/04 , C12P21/06 , C07K14/195
CPC分类号: C07H15/252 , C07H17/08 , C12N15/52 , C12P19/18
摘要: Hybrid glycosylated products such as polyketides and peptides are produced by transforming a host cell with (a) a gene cassette for synthesising an activated sugar and (b) nucleic acid encoding a glycosyltransferase (GT). The cell also produces or is supplied with an aglycone template. At least some of the components (sugar, aglycone, GT, sugar synthesis genes, cells) are mutually heterologous.
摘要翻译: 通过用(a)用于合成活性糖的基因盒和(b)编码糖基转移酶(GT)的核酸转化宿主细胞来产生杂合糖基化产物如聚酮化合物和肽。 细胞还生产或提供糖苷配基模板。 至少一些组分(糖,糖苷配基,GT,糖合成基因,细胞)是相互异源的。