公开(公告)号：US20180016571A1

公开(公告)日：2018-01-18

申请号：US15523765

申请日：2015-11-05

Applicant: Illumina, Inc.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US12180468B2

公开(公告)日：2024-12-31

申请号：US17010349

申请日：2020-09-02

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US10815478B2

公开(公告)日：2020-10-27

申请号：US15523765

申请日：2015-11-05

Applicant: Illumina, Inc.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US20210047635A1

公开(公告)日：2021-02-18

申请号：US17010349

申请日：2020-09-02

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US20250145985A1

公开(公告)日：2025-05-08

申请号：US18952092

申请日：2024-11-19

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US11118175B2

公开(公告)日：2021-09-14

申请号：US16210284

申请日：2018-12-05

Applicant: Illumina, Inc.

Inventor： Haiying Li Grunenwald ,  Nicholas Caruccio ,  Jerome Jendrisak ,  Gary Dahl

IPC: C12N15/10 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6855 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing so-called “next generation sequencing”).