公开(公告)号：US11781182B2

公开(公告)日：2023-10-10

申请号：US16929632

申请日：2020-07-15

Applicant: ILLUMINA, INC.

Inventor： Erin Bomati ,  Molly He ,  Cheng-Yao Chen ,  Sergio Peisajovich

IPC: C12N9/12 ,  C12Q1/6844 ,  C12Q1/6869

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Q1/6844 ,  C12Y207/07007 ,  C12Q1/6844 ,  C12Q2521/101

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：US20230295701A1

公开(公告)日：2023-09-21

申请号：US18063865

申请日：2022-12-09

Applicant: Illumina, Inc.

Inventor： Jeffrey G. Mandell ,  Molly He

IPC: C12Q1/6844 ,  C12Q1/686

CPC classification number: C12Q1/6844 ,  C12Q1/686

Abstract: A method for enriching or amplifying a target nucleic acid including providing a system having a guide nucleic acid, and a Cas or Argonaute protein or a variant thereof. The guide nucleic acid contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid, and contacting the target nucleic acid with the system to form a complex.

公开(公告)号：US20230212534A1

公开(公告)日：2023-07-06

申请号：US18085674

申请日：2022-12-21

Applicant: ILLUMINA, INC. ,  ILLUMINA SINGAPORE PTE LTD

Inventor： Misha Golynskiy ,  Seth McDonald ,  Saurabh Nirantar ,  Matthew Kellinger ,  Michael Previte ,  Sergio Peisajovich ,  Molly He

IPC: C12N9/12 ,  C12N1/20

CPC classification number: C12N9/1241 ,  C12N1/20

Abstract: Presented herein are altered polymerase enzymes for improved incorporation of nucleotides and nucleotide analogues, in particular altered polymerases that maintain high fidelity under reduced incorporation times, as well as methods and kits using the same.

公开(公告)号：US11242560B2

公开(公告)日：2022-02-08

申请号：US16127067

申请日：2018-09-10

Applicant: Illumina, Inc.

Inventor： Michael Previte ,  Molly He ,  Rigo Pantoja ,  Cheng-Yao Chen ,  Chunhong Zhou

IPC: C12Q1/6874

Abstract: A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

公开(公告)号：US20210047635A1

公开(公告)日：2021-02-18

申请号：US17010349

申请日：2020-09-02

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US10385323B2

公开(公告)日：2019-08-20

申请号：US16021417

申请日：2018-06-28

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Erin Bomati ,  Molly He ,  Haiying Li Grunenwald ,  Scott Kuersten ,  Trina Faye Osothprarop ,  Darin Haskins ,  Joshua Burgess ,  Anupama Khanna ,  Daniel Schlingman ,  Ramesh Vaidyanathan

IPC: C12N9/12 ,  C12N9/22 ,  C12N15/10 ,  C12Q1/6869

Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

公开(公告)号：US10150954B2

公开(公告)日：2018-12-11

申请号：US15610875

申请日：2017-06-01

Applicant: Illumina, Inc.

Inventor： Erin Bomati ,  Michael Previte ,  Matthew William Kellinger ,  Cheng-Yao Chen ,  Molly He

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/686

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogs, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：US09790476B2

公开(公告)日：2017-10-17

申请号：US14686961

申请日：2015-04-15

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Erin Bomati ,  Molly He ,  Haiying Li Grunenwald ,  Scott Kuersten ,  Trina Faye Osothprarop ,  Darin Haskins ,  Joshua Burgess ,  Anupama Khanna ,  Daniel Schlingman ,  Ramesh Vaidyanathan

IPC: C12N9/12 ,  C12N9/22 ,  C12N15/10 ,  C12Q1/68

CPC classification number: C12N9/1241 ,  C12N9/22 ,  C12N15/1068 ,  C12Q1/6869 ,  C12Q2521/507 ,  C12Q2565/514

Abstract: Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.

公开(公告)号：US20250145985A1

公开(公告)日：2025-05-08

申请号：US18952092

申请日：2024-11-19

Applicant: ILLUMINA, INC.

Inventor： Christian Gloeckner ,  Amirali Kia ,  Molly He ,  Trina Faye Osothprarop ,  Frank J. Steemers ,  Kevin L. Gunderson ,  Sasan Amini ,  Jerome Jendrisak

IPC: C12N15/10 ,  C12N9/12

Abstract: Presented herein are methods and compositions for tagmentation of nucleic acids. The methods are useful for generating tagged DNA fragments that are qualitatively and quantitatively representative of the target nucleic acids in the sample from which they are generated.

公开(公告)号：US20240003057A1

公开(公告)日：2024-01-04

申请号：US18368496

申请日：2023-09-14

Applicant: Illumina, Inc.

Inventor： Molly He ,  Michael Previte ,  Misha Golynskiy ,  Matthew William Kellinger ,  Sergio Peisajovich ,  Jonathan Mark Boutell

IPC: C40B50/16 ,  B01L3/00 ,  B01J19/00 ,  C40B20/04 ,  C12N15/10

CPC classification number: C40B50/16 ,  B01L3/5085 ,  B01J19/0046 ,  B01L3/54 ,  C40B20/04 ,  C12N15/1062 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/1068 ,  B01L2300/021 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00626 ,  B01J2219/00743

Abstract: A method of characterizing candidate agents including steps of (a) providing a library of candidate agents attached to nucleic acid tags; (b) contacting the library with a solid support to attach the candidate agents to the solid support, whereby an array of candidate agents is formed; (c) contacting the array with a screening agent, wherein one or more candidate agents in the array react with the screening agent; (d) detecting the array to determine that at least one candidate agent in the array reacts with the screening agent; (e) sequencing the nucleic acid tag to determine the tag sequences attached to candidate agents in the array; and (f) identifying the at least one candidate agent in the array that reacts with the screening agent based on the tag sequence that is attached to the at least one candidate agent.