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公开(公告)号:US20190352327A1
公开(公告)日:2019-11-21
申请号:US16411913
申请日:2019-05-14
发明人: Xiaolin Wu , Randall Smith , Peyton Shieh , John M. Beierle , Wayne N. George , Elliot John Lawrence , Jie Mao , Xiaohai Liu
IPC分类号: C07H21/04 , C12Q1/6876
摘要: Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.