-
公开(公告)号：US20220145379A1
公开(公告)日：2022-05-12
申请号：US17599994
申请日：2020-11-25
发明人： Wayne N. George , Brian D. Mather , Andrew A. Brown , Pietro Gatti Lafranconi , Maria Candelaria Rogert Bacigalupo , Antoine Francais , Xiaohai Liu
IPC分类号： C12Q1/6848 , C12Q1/6869
摘要： An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.
-
2.发明申请公开(公告)号：US20190352327A1
公开(公告)日：2019-11-21
申请号：US16411913
申请日：2019-05-14
发明人： Xiaolin Wu , Randall Smith , Peyton Shieh , John M. Beierle , Wayne N. George , Elliot John Lawrence , Jie Mao , Xiaohai Liu
IPC分类号： C07H21/04 , C12Q1/6876
摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
-
公开(公告)号：US11293061B2
公开(公告)日：2022-04-05
申请号：US16724088
申请日：2019-12-20
发明人： Antoine Francais , Elena Cressina , Adam Culley , Angelica Mariani , Xiaolin Wu , Xiaohai Liu
IPC分类号： C12Q1/68 , C12Q1/6869 , C07H19/06 , C07H19/16 , C07H21/00
摘要： Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3′ AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.
-
公开(公告)号：US11028116B2
公开(公告)日：2021-06-08
申请号：US17035053
申请日：2020-09-28
发明人： Xiaohai Liu , John Milton , Silke Ruediger , Xiaolin Wu
IPC分类号： C12Q1/68 , C07H19/04 , C07H19/16 , C07H19/20 , C07H19/14 , C07H19/06 , C07H21/00 , C07H19/10 , C12Q1/6869 , C07H21/04 , C12Q1/6874
摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
-
公开(公告)号：US11008359B2
公开(公告)日：2021-05-18
申请号：US16916887
申请日：2020-06-30
发明人： Xiaohai Liu , John Milton , Silke Ruediger , Xiaolin Wu
IPC分类号： C07H19/04 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C07H21/04 , C12Q1/6869 , C12Q1/6874 , C12Q1/68
摘要： The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (I) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C1-10 substituted or unsubstituted alkyl group, Y is selected from the group comprising O, S, NH and N(allyl), T is hydrogen or a C1-10 substituted or unsubstituted alkyl group and * indicates where the moiety is connected to the remainder of the nucleotide or nucleoside).
-
公开(公告)号：US10480025B2
公开(公告)日：2019-11-19
申请号：US15202456
申请日：2016-07-05
发明人： Shankar Balasubramanian , Colin Lloyd Barnes , Xiaohai Liu , John Milton , Xiaolin Wu , Harold Swerdlow
IPC分类号： C07H21/00 , C12Q1/6869 , C07H19/10 , C07H19/20 , C12P19/34 , C12Q1/6876 , C12Q1/6874 , C12Q1/6818 , C12Q1/6834 , C12Q1/6806 , C12Q1/6823 , C12Q1/68
摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
-
公开(公告)号：US20190153529A1
公开(公告)日：2019-05-23
申请号：US16258401
申请日：2019-01-25
发明人： Xiaolin Wu , Xiaohai Liu
IPC分类号： C12Q1/6869 , C07H19/14
摘要： Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.
-
公开(公告)号：US20180353926A1
公开(公告)日：2018-12-13
申请号：US16010902
申请日：2018-06-18
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
IPC分类号： B01J19/00 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
公开(公告)号：US20180282791A1
公开(公告)日：2018-10-04
申请号：US15762073
申请日：2016-09-26
发明人： Antoine Francais , Xiaohai Liu
IPC分类号： C12Q1/6816 , C09B23/06 , C09B23/10
摘要： The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
-
公开(公告)号：US09999866B2
公开(公告)日：2018-06-19
申请号：US15287077
申请日：2016-10-06
发明人： Xiaohai Liu , John Milton , Geoffrey Paul Smith , Colin Lloyd Barnes , Isabelle Rasolonjatovo , Roberto Rigatti , Xiaolin Wu , Tobias William Barr Ost , Graham John Worsley , David James Earnshaw , Gerardo Turcatti , Anthony Romieu
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5′ end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
-
-
-
-
-
-
-
-
-
搜索结果
70 条记录 (耗时 0.051 秒)