公开(公告)号：US11124761B2

公开(公告)日：2021-09-21

申请号：US16212212

申请日：2018-12-06

Applicant: Indiana University Research and Technology Corporation

Inventor： Chien-Chi Lin ,  Camron Scott Dawes ,  Heiko Konig

IPC: C12N5/00 ,  G01N33/50 ,  C12M1/12 ,  C12M1/00

Abstract: Embodiments of the current invention include a hydrogel formed from crosslinked polyethylene glycol into which acrylated glucose oxidase has been immobilized through crosslinking to the gel. These hydrogels can be used to create hypoxia under ambient conditions for at least 72 hours and can be used to create hypoxic gradients. These embodiments permit the study of cells under a variety of hypoxic conditions.

公开(公告)号：US20190177683A1

公开(公告)日：2019-06-13

申请号：US16212212

申请日：2018-12-06

Applicant: Indiana University Research and Technology Corporation

Inventor： Chien-Chi Lin ,  Camron Scott Dawes ,  Heiko Konig

IPC: C12N5/00 ,  G01N33/50 ,  C12M1/12

Abstract: Embodiments of the current invention include a hydrogel formed from crosslinked polyethylene glycol into which acrylated glucose oxidase has been immobilized through crosslinking to the gel. These hydrogels can be used to create hypoxia under ambient conditions for at least 72 hours and can be used to create hypoxic gradients. These embodiments permit the study of cells under a variety of hypoxic conditions.