公开(公告)号：US10196600B2

公开(公告)日：2019-02-05

申请号：US14736363

申请日：2015-06-11

Applicant: Industrial Technology Research Institute

Inventor： Ming-Der Bai ,  Chung-Cheng Han ,  Wen-Chang Lu ,  Hou-Peng Wan ,  Jo-Shu Chang ,  Chun-Yen Chen ,  Hsin-Yueh Chang

IPC: C12N1/06 ,  C12N1/20 ,  C12P1/04 ,  C12R1/07

Abstract: The disclosure provides a method for manufacturing an active substance for inducing self-lysis in microalga cells, including: inoculating a bacterial strain belonging to Bacillus into a culturing medium to obtain a bacterial suspension; culturing the bacterial strain belonging to Bacillus at least to a stationary phase to a condition in which the bacterial strain belonging to Bacillus aggregates in the bacterial suspension and the bacterial suspension becomes pellucid; and after the bacterial strain belonging to Bacillus aggregates in the bacterial suspension and the bacterial suspension becomes pellucid, performing a vacuum distillation procedure on the bacterial suspension to obtain an active solution, wherein the active solution contains an active substance for inducing self-lysis in microalga cells.