公开(公告)号：US10112969B2

公开(公告)日：2018-10-30

申请号：US15589176

申请日：2017-05-08

Applicant: Industrial Technology Research Institute

Inventor： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

IPC: C07H21/02 ,  C07H21/04 ,  C07H21/00 ,  C12Q1/6869 ,  C12Q1/68 ,  C12P19/34

Abstract: Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.

公开(公告)号：US20180111959A1

公开(公告)日：2018-04-26

申请号：US15589176

申请日：2017-05-08

Applicant: Industrial Technology Research Institute

Inventor： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

IPC: C07H21/04 ,  C12Q1/6869

CPC classification number: C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2525/119 ,  C12Q2565/101

Abstract: Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.