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公开(公告)号：US10112969B2
公开(公告)日：2018-10-30
申请号：US15589176
申请日：2017-05-08
Applicant: Industrial Technology Research Institute
Inventor： Chung-Fan Chiou , Chao-Chi Pan , Jenn-Yeh Fann , Shang-Chia Chang , Tsu-Ju Fu
Abstract: Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.
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2.发明申请公开(公告)号：US20140256570A1
公开(公告)日：2014-09-11
申请号：US14286904
申请日：2014-05-23
Applicant: Industrial Technology Research Institute
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
CPC classification number: G06F19/22 , C12Q1/6869 , C12Q2531/125 , C12Q2537/165
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
Abstract translation: 本文公开了在具有已知序列的核酸插入物的环状分子中存在的核酸样品中修饰碱基的序列和/或位置的方法，包括获得至少两个插入样品单元的序列数据。 在一些实施方案中，所述方法包括使用环状双锁定分子获得序列数据。 在一些实施方案中，所述方法包括通过将序列与核酸插入物的已知序列进行比较来计算核酸插入序列的分数，以及根据一个或多个核酸插入片段的得分接受或排除核酸样品序列的重复序列 插入物的两个序列紧接着核酸样品序列的重复序列的上游或下游。
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公开(公告)号：US10515714B2
公开(公告)日：2019-12-24
申请号：US15659585
申请日：2017-07-25
Applicant: Industrial Technology Research Institute
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
IPC: C12Q1/68 , G16B30/00 , C12Q1/6869
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
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公开(公告)号：US20130230909A1
公开(公告)日：2013-09-05
申请号：US13889298
申请日：2013-05-07
Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
IPC: G06F19/22
CPC classification number: G16B30/00 , C12Q1/6869 , C12Q2531/125 , C12Q2537/165
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
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公开(公告)号：US20180111959A1
公开(公告)日：2018-04-26
申请号：US15589176
申请日：2017-05-08
Applicant: Industrial Technology Research Institute
Inventor： Chung-Fan Chiou , Chao-Chi Pan , Jenn-Yeh Fann , Shang-Chia Chang , Tsu-Ju Fu
IPC: C07H21/04 , C12Q1/6869
CPC classification number: C07H21/00 , C07H21/04 , C12Q1/6869 , C12Q2525/117 , C12Q2525/119 , C12Q2565/101
Abstract: Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.
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Patent Agency Ranking
公开(公告)号：US20170009286A1
公开(公告)日：2017-01-12
申请号：US15260773
申请日：2016-09-09
Applicant: Industrial Technology Research Institute
Inventor： Chung-Fan Chiou , Ying-Chih Pu , Chao-Chi Pan , Chih-Tsung Shih , Ming-Chia Li , Chein-Shiu Kuo , Hung-Chi Chien , Chang-Sheng Chu
CPC classification number: C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/6454 , G01N21/648 , G01N21/7703 , G01N2021/6439 , G01N2201/08 , G01N2201/12 , C12Q2563/155 , C12Q2561/113
Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.
Abstract translation: 实施例包括单分子检测系统和使用检测系统检测物体的方法。 此外，实施例包括包括可移动光耦合器，波导和光检测器的检测系统。 实施方案进一步包括单分子检测的方法，包括单分子核酸测序的方法。
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公开(公告)号：US09767251B2
公开(公告)日：2017-09-19
申请号：US14717996
申请日：2015-05-20
Applicant: Industrial Technology Research Institute
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
CPC classification number: G06F19/22 , C12Q1/6869 , C12Q2531/125 , C12Q2537/165
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
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公开(公告)号：US11676682B1
公开(公告)日：2023-06-13
申请号：US16672232
申请日：2019-11-01
Applicant: Industrial Technology Research Institute
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
IPC: C12Q1/68 , C12Q1/6869 , G16B30/00
CPC classification number: G16B30/00 , C12Q1/6869
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
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公开(公告)号：US09777321B2
公开(公告)日：2017-10-03
申请号：US15260773
申请日：2016-09-09
Applicant: Industrial Technology Research Institute
Inventor： Chung-Fan Chiou , Ying-Chih Pu , Chao-Chi Pan , Chih-Tsung Shih , Ming-Chia Li , Chein-Shiu Kuo , Hung-Chi Chien , Chang-Sheng Chu
CPC classification number: C12Q1/6869 , G01N21/6428 , G01N21/6452 , G01N21/6454 , G01N21/648 , G01N21/7703 , G01N2021/6439 , G01N2201/08 , G01N2201/12 , C12Q2563/155 , C12Q2561/113
Abstract: Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.
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公开(公告)号：US09747414B2
公开(公告)日：2017-08-29
申请号：US14286904
申请日：2014-05-23
Applicant: Industrial Technology Research Institute
Inventor： Chao-Chi Pan , Jenn-Yeh Fann , Chung-Fan Chiou , Hung-Chi Chien , Hui-Ling Chen
CPC classification number: G06F19/22 , C12Q1/6869 , C12Q2531/125 , C12Q2537/165
Abstract: Disclosed herein are methods of determining the sequence and/or positions of modified bases in a nucleic acid sample present in a circular molecule with a nucleic acid insert of known sequence comprising obtaining sequence data of at least two insert-sample units. In some embodiments, the methods comprise obtaining sequence data using circular pair-locked molecules. In some embodiments, the methods comprise calculating scores of sequences of the nucleic acid inserts by comparing the sequences to the known sequence of the nucleic acid insert, and accepting or rejecting repeats of the sequence of the nucleic acid sample according to the scores of one or both of the sequences of the inserts immediately upstream or downstream of the repeats of the sequence of the nucleic acid sample.
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