公开(公告)号：US20180230460A1

公开(公告)日：2018-08-16

申请号：US15948793

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB ,  Marcelo Colika BASSALO ,  Ramsey Ibrahim ZEITOUN

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20180230461A1

公开(公告)日：2018-08-16

申请号：US15948798

申请日：2018-04-09

Applicant: The Regents of the University of Colorado, a body corporate ,  Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB ,  Marcelo Colika BASSALO ,  Ramsey Ibrahim ZEITOUN

IPC: C12N15/10 ,  C12N15/11

CPC classification number: C12N15/1065 ,  C12N15/102 ,  C12N15/1079 ,  C12N15/1082 ,  C12N15/11 ,  C12N2310/20 ,  C12Q2563/179

Abstract: Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.

公开(公告)号：US20190390226A1

公开(公告)日：2019-12-26

申请号：US16548631

申请日：2019-08-22

Applicant: Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB

IPC: C12N15/90 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N9/22 ,  C12N15/11

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.

公开(公告)号：US20180371498A1

公开(公告)日：2018-12-27

申请号：US15896444

申请日：2018-02-14

Applicant: Inscripta, Inc.

Inventor： Ryan T. GILL ,  Andrew GARST ,  Tanya Elizabeth Warnecke LIPSCOMB

IPC: C12N15/90 ,  C12N15/11 ,  C12N15/85 ,  C12N15/70 ,  C12N9/22 ,  C12N15/81

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/102 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/81 ,  C12N15/85 ,  C12N15/905 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/22

Abstract: Disclosed herein are nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Disclosed herein are engineered non-naturally occurring nucleic acid-guided nucleases, guide nucleic acids, and targetable nuclease systems, and methods of use. Targetable nuclease systems can be used to edit genetic targets, including recursive genetic engineering and trackable genetic engineering methods.