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    Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines
    2.
    发明授权
    Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines 失效
    在胺存在下，以高蛋白浓度重组含二硫键的蛋白质复性的方法

    公开(公告)号：US07893210B2

    公开(公告)日：2011-02-22

    申请号：US10495417

    申请日：2002-11-12

    申请人： Jörg Peters ,  Torsten Minuth

    发明人： Jörg Peters ,  Torsten Minuth

    IPC分类号： C07K14/54 ,  C07K14/52 ,  C07K14/435 ,  C07K14/00

    CPC分类号： C07K1/1133 ,  C07K14/5406 ,  C07K14/8117

    摘要： A method for renaturation of proteins comprising adding to a solution of denatured, chemically modified or reduced proteins a refolding buffer containing a primary, secondary or tertiary amine. Said method has been applied, for example, to interleukin-4 and bovine pancreatic trypsin inhibitor (BPTI), which were previously (i) solubilized in the presence of guanidinium hydrochloride as chaotronic agent, and (ii) subjected to sulfitolysis.

    摘要翻译： 一种蛋白质复性的方法，包括向变性，化学修饰或还原蛋白质的溶液中加入含有伯胺，仲胺或叔胺的重折叠缓冲液。 所述方法已经应用于例如以前（i）在盐酸胍盐溶解作为混合剂的白细胞介素-4和牛胰蛋白酶抑制剂（BPTI）中，和（ii）进行亚硫酸解。

    Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines
    3.
    发明申请
    Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines 失效
    在胺存在下，以高蛋白浓度重组含二硫键的蛋白质复性的方法

    公开(公告)号：US20050014933A1

    公开(公告)日：2005-01-20

    申请号：US10495417

    申请日：2002-11-12

    申请人： Jörg Peters ,  Torsten Minuth

    发明人： Jörg Peters ,  Torsten Minuth

    IPC分类号： C12P21/02 ,  C07K1/113 ,  C07K14/54 ,  C07K14/81

    CPC分类号： C07K1/1133 ,  C07K14/5406 ,  C07K14/8117

    摘要： A method for renaturation of proteins comprising adding to a solution of denatured, chemically modified or reduced proteins a refolding buffer containing a primary, secondary or tertiary amine. Said method has been applied, for example, to interleukin-4 and bovine pancreatic trypsin inhibitor (BPTI), wich were previously (i) solubilized in the presence of guanidinium hydrochloride as chaotronic agent, and (ii) subjected to sulfitolysis.

    摘要翻译： 一种蛋白质复性的方法，包括向变性，化学修饰或还原蛋白质的溶液中加入含有伯胺，仲胺或叔胺的重折叠缓冲液。 所述方法已经应用于例如以前（i）在盐酸胍盐溶液中溶解的白细胞介素-4和牛胰蛋白酶抑制剂（BPTI），并且（ii）进行亚硫酸解。

    Purification of protein inclusion bodies by crossflow microfiltration
    6.
    发明授权
    Purification of protein inclusion bodies by crossflow microfiltration 失效
    通过交叉流微滤法纯化蛋白质包涵体

    公开(公告)号：US06936699B2

    公开(公告)日：2005-08-30

    申请号：US10275797

    申请日：2001-04-25

    申请人： Jörg Peters

    发明人： Jörg Peters

    IPC分类号： C12N1/00 ,  C07K1/34 ,  A23J1/00 ,  C12P21/06

    CPC分类号： C07K1/34

    摘要： The invention relates to a process for purifying and/or concentrating inclusion bodies (refractile bodies) in a highly concentrated particle-containing solution, in which process the inclusion body-containing suspension is directed tangentially past one or more semipermeable membranes, so that the inclusion bodies are retained behind the membranes and that substances having a relatively small molecular weight may pass through the membrane and/or be adsorbed to the membrane, and a purified and/or concentrated inclusion body suspension is obtained. In the same manner, cell wall particles are removed from a inclusion body suspension. The invention further relates to the use of a crossflow microfiltration unit for purifying and/or concentrating inclusion bodies in a suspension and for removing cell wall particles from a inclusion body preparation.

    摘要翻译： 本发明涉及一种用于在高浓度含有颗粒的溶液中净化和/或浓缩包涵体（折射体）的方法，其中含有夹杂物的悬浮液切向地穿过一个或多个半透膜，使得包含 身体保留在膜的后面，并且具有相对较小分子量的物质可以通过膜和/或吸附到膜上，并且获得纯化和/或浓缩的包涵体悬浮液。 以相同的方式，从包涵体悬浮液中除去细胞壁颗粒。 本发明还涉及使用交叉流微量微滤单元来净化和/或浓缩悬浮液中的包涵体和从包涵体制剂中除去细胞壁颗粒。