公开(公告)号：US5872357A

公开(公告)日：1999-02-16

申请号：US866724

申请日：1997-05-30

申请人： J. Michael Flanagan

发明人： J. Michael Flanagan

IPC分类号： C07F9/6593 ,  H01J49/04 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/0009 ,  C07F9/65815

摘要： Calibrant compositions are provided for use in either electrospray or APCI mass spectrometry, and can be used in either positive-ionization or negative-ionization modes. The compositions contain a primary calibrant preferably comprised of a mixture of homogeneously substituted triazatriphosphorines, and a secondary calibrant enabling calibration at relatively low mass-to-charge (m/z) values. The compositions enable calibration across a broad mass spectral range. Novel triazatriphosphorine compounds are provided as well, as are methods for calibrating mass spectrometers using the novel calibrant compositions.

摘要翻译： 提供校准剂组合物用于电喷雾或APCI质谱法，并且可以用于正离子化或负电离模式。 组合物含有主要的校准物优选由均匀取代的三氮杂三肽的混合物组成，并且能够以相对较低的质荷比（m / z）值进行校准的二次校准剂。 该组合物能够在宽的质谱范围内进行校准。 还提供了新的三氮杂磷酸化合物，以及使用新型校准物组合物校准质谱仪的方法。

公开(公告)号：US06849847B1

公开(公告)日：2005-02-01

申请号：US09146817

申请日：1998-09-04

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： H01J49/04 ,  H01J49/16 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于递送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子递送到质量分析装置的通道;（b）将所述电离外壳保持在大于100mTorr的环境压力 （c）保持器，其构造成用于在所述环境压力下将包含所述样品的基质保持在电离外壳中;（d）激光能量源，包括与电离外壳相关联的装置，用于将激光能量引导到由保持器保持的所述基体上 在环境压力下解吸并离子化样品中的至少一部分分析物，和（e）用于引导至少一个i的至少一部分的装置 通入分析物。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US07193206B2

公开(公告)日：2007-03-20

申请号：US10806907

申请日：2004-03-22

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises: The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于传送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子输送到质量分析装置的通道; （b）将所述电离外壳保持在大于100mTorr的环境压力下的装置; （c）构造成用于在所述环境压力下将包含所述样品的基质保持在所述电离外壳中的保持器; （d）激光能量源，其包括与电离外壳相关联的装置，用于将激光能量引导到由保持器在环境压力下维持的所述基体上以解吸并离子化样品中的至少一部分分析物，以及（e） 用于将所述至少一种离子化分析物的至少一部分引导到所述通道中的装置。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US07102128B2

公开(公告)日：2006-09-05

申请号：US10806808

申请日：2004-03-22

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

公开(公告)号：US08338780B2

公开(公告)日：2012-12-25

申请号：US10806829

申请日：2004-03-22

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： H01J49/16 ,  H01J49/04

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于传送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子输送到质量分析装置的通道; （b）将所述电离外壳保持在大于100mTorr的环境压力下的装置; （c）构造成用于在所述环境压力下将包含所述样品的基质保持在所述电离外壳中的保持器; （d）激光能量源，其包括与电离外壳相关联的装置，用于将激光能量引导到由保持器在环境压力下维持的所述基体上以解吸并离子化样品中的至少一部分分析物，以及（e） 用于将所述至少一种离子化分析物的至少一部分引导到所述通道中的装置。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US06989530B2

公开(公告)日：2006-01-24

申请号：US10806685

申请日：2004-03-22

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising: (a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device; (b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr; (c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure; (d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and (e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于传送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子输送到质量分析装置的通道; （b）将所述电离外壳保持在大于100mTorr的环境压力下的装置; （c）构造成用于在所述环境压力下将包含所述样品的基质保持在所述电离外壳中的保持器; （d）激光能量源，其包括与电离外壳相关联的装置，用于将激光能量引导到由保持器在环境压力下维持的所述基体上以解吸并离子化样品中的至少一部分分析物，以及（e） 用于将所述至少一种离子化分析物的至少一部分引导到所述通道中的装置。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US06952012B2

公开(公告)日：2005-10-04

申请号：US10806908

申请日：2004-03-22

申请人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

发明人： Jian Bai ,  Steven M. Fischer ,  J. Michael Flanagan

IPC分类号： H01J49/04 ,  H01J49/16 ,  B01D59/44 ,  H01J49/00

CPC分类号： H01J49/164

摘要： A mass spectrometer having a matrix-assisted laser desorption ionization (MALDI) source which operates at ambient pressure is disclosed. The apparatus and method are disclosed to analyze at least one sample which contains at least one analyte using matrix-assisted laser desorption ionization (MALDI), which apparatus comprises:The present invention relates to an apparatus and a method for ionizing at least one analyte in a sample for delivery to a mass analysis device, comprising:(a) an ionization enclosure including a passageway configured for delivery of ions to the mass analysis device;(b) means to maintain said ionization enclosure at an ambient pressure of greater than 100 mTorr;(c) a holder configured for maintaining a matrix containing said sample in the ionization enclosure at said ambient pressure;(d) a source of laser energy including means associated with the ionization enclosure for directing the laser energy onto said matrix maintained by the holder at the ambient pressure to desorb and ionize at least a portion of the analyte in the sample, and(e) means for directing at least a portion of the at least one ionized analyte into the passageway. The ambient pressure (AP-MALDI) source is compatible with various mass analyzers, particularly with mass spectrometers and solves many problems associated with conventional MALDI sources operating under vacuum. Atmospheric pressure MALDI is described. The analysis of organic molecules or fragments thereof, particularly biomolecules, e.g., biopolymers and organisms, is described.

摘要翻译： 公开了具有在环境压力下操作的基质辅助激光解吸电离（MALDI）源的质谱仪。 公开了该装置和方法以使用矩阵辅助激光解吸电离（MALDI）分析至少一个含有至少一种分析物的样品，该装置包括：本发明涉及用于将至少一种分析物离子化的装置和方法 用于传送到质量分析装置的样品，包括：（a）电离外壳，其包括经配置以将离子输送到质量分析装置的通道; （b）将所述电离外壳保持在大于100mTorr的环境压力下的装置; （c）构造成用于在所述环境压力下将包含所述样品的基质保持在所述电离外壳中的保持器; （d）激光能量源，其包括与电离外壳相关联的装置，用于将激光能量引导到由保持器在环境压力下维持的所述基体上以解吸并离子化样品中的至少一部分分析物，以及（e） 用于将所述至少一种离子化分析物的至少一部分引导到所述通道中的装置。 环境压力（AP-MALDI）源与各种质量分析仪兼容，特别是与质谱仪兼容，解决了与在真空下运行的常规MALDI源有关的许多问题。 描述了大气压力MALDI。 描述了有机分子或其片段，特别是生物分子，例如生物聚合物和生物体的分析。

公开(公告)号：US5726447A

公开(公告)日：1998-03-10

申请号：US679647

申请日：1996-07-12

申请人： Edward Aisawa ,  James L. Bertsch ,  J. Michael Flanagan ,  Darrell L. Gourley ,  Val C. Robinson ,  Joseph Vandenburg

发明人： Edward Aisawa ,  James L. Bertsch ,  J. Michael Flanagan ,  Darrell L. Gourley ,  Val C. Robinson ,  Joseph Vandenburg

IPC分类号： H01J49/04 ,  H01J49/10

CPC分类号： H01J49/145

摘要： The invention relates to an ionization chamber. More particularly, the invention relates to a mass spectrometer system having an ionization chamber incorporating a corona needle removable from outside the closed ionization chamber. In preferred embodiments, the corona needle is self-positioning and engages and disengages operational or safety electrical connections.

摘要翻译： 本发明涉及电离室。 更具体地说，本发明涉及一种质谱仪系统，其具有电离室，该离子化室包含从封闭电离室外部可去除的电晕针。 在优选实施例中，电晕针是自定位的，并接合和分离操作或安全的电连接。