公开(公告)号：US20170022528A1

公开(公告)日：2017-01-26

申请号：US15265123

申请日：2016-09-14

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kazunobu KONISHI ,  Naoki TAKAYA ,  Shunsuke MASUO

IPC: C12P13/22 ,  C12P13/00

CPC classification number: C12P13/222 ,  C12P13/001 ,  C12P13/005 ,  C12P13/22

Abstract: Provided is a method for producing an aniline derivative by fermentation from a carbon source such as glucose. The method comprises the following steps: production of microorganisms capable of producing 1.8 g/L or more of 4-aminophenylalanine (4APhe) under prescribed culture conditions by introducing at least three exogenous genes into microorganisms having the ability to biosynthesize 4-aminophenylpyruvic acid from chorismic acid; and production of at least one aniline derivative selected from the group consisting of 4-aminophenylalanine (4APhe), 4-aminocinnamic acid (4ACA), 2-(4-aminophenyl)aldehyde, 4-aminophenylacetic acid, and 4-aminophenethylethanol (4APE) by bringing these microorganisms into contact with a carbon source under conditions suited to the growth and/or maintenance of these microorganisms.

Abstract translation: 本发明提供了通过葡萄糖等碳源的发酵生产苯胺衍生物的方法。 该方法包括以下步骤：在规定的培养条件下生产能够产生1.8g / L或更多的4-氨基苯丙氨酸（4APhe）的微生物，通过将至少三种外源基因引入到具有生物合成4-氨基苯基丙酮酸的能力的微生物中 酸; 选自4-氨基苯丙氨酸（4APhe），4-氨基肉桂酸（4ACA），2-（4-氨基苯基）醛，4-氨基苯基乙酸和4-氨基苯乙醇（4APE）的至少一种苯胺衍生物的制备， 通过使这些微生物在适合于这些微生物的生长和/或维持的条件下与碳源接触。

公开(公告)号：US20210017555A1

公开(公告)日：2021-01-21

申请号：US16976222

申请日：2019-03-04

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Naoki TAKAYA ,  Shunsuke MASUO ,  Yukie KAWASAKI ,  Hajime MINAKAWA

IPC: C12P13/00

Abstract: Provided is a novel method for producing 4-aminocinnamic acid from 4-nitrophenylalanine. This method comprises: converting 4-nitrophenylalanine into 4-nitrocinnamic acid; and converting 4-nitrocinnamic acid into 4-aminocinnamic acid.

公开(公告)号：US20160362711A1

公开(公告)日：2016-12-15

申请号：US15229661

申请日：2016-08-05

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Kazunobu KONISHI ,  Naoki TAKAYA ,  Shunsuke MASUO ,  Shengmin ZHOU

IPC: C12P13/00 ,  C12N9/88 ,  C07C229/44 ,  C12N15/70

CPC classification number: C12P13/005 ,  C07C229/44 ,  C12N9/88 ,  C12N15/70 ,  C12Y403/01024

Abstract: This 4-amino cinnamic acid production method using the enzyme ammonia lyase can efficiently convert 4-amino phenylalanine into 4-amino cinnamic acid. This 4-amino cinnamic acid production method is characterized by converting 4-amino phenylalanine into 4-amino cinnamic acid by using phenylalanine ammonia-lyase, which comprises the amino acid sequence represented in sequence number 2 derived from the Rhodotorula glutinis yeast.

Abstract translation: 使用酶氨裂解酶的4-氨基肉桂酸生产方法可以有效地将4-氨基苯丙氨酸转化为4-氨基肉桂酸。 该4-氨基肉桂酸生产方法的特征在于使用苯丙氨酸氨裂解酶将4-氨基苯丙氨酸转化成4-氨基肉桂酸，其包含源自红酵母（Rhodotorula glutinis）酵母的序列号2所示的氨基酸序列。