公开(公告)号：US20190256576A1

公开(公告)日：2019-08-22

申请号：US14912444

申请日：2014-08-13

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Taizo UDA ,  Emi Hifumi

IPC: C07K16/00

Abstract: A human antibody κ type light chain complex-containing composition includes a complex in which a human antibody κ type light chain is bound to one or more kinds of metal ions selected from the group consisting of Group 10 elements, Group 11 elements, and Group 12 elements. The human antibody κ type light chain is a dimer, cysteines at C terminals of two human antibody κ type light chains are bound to each other via the metal ion, and 0.1 mol or more of the metal ion is bound per 1 mol of the human antibody κ type light chain.

公开(公告)号：US20220259290A1

公开(公告)日：2022-08-18

申请号：US17627337

申请日：2020-07-22

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Taizo UDA ,  Emi HIFUMI

IPC: C07K16/06 ,  C07K1/16

Abstract: A method for producing a κ light chain antibody having enzyme activity or improved enzyme activity includes modifying a polynucleotide that encodes a κ light chain antibody having a polypeptide having an amino acid sequence where the 95th amino acid residue from the N-terminal of a variable region by the Kabat classification is a proline residue, to delete or substitute the proline residue and to obtain a polynucleotide that encodes a κ light chain antibody having a polypeptide having an amino acid sequence where the 95th amino acid residue from the N-terminal of a variable region by the Kabat classification is deleted or substituted with an amino acid residue other than a proline residue, and expressing a κ light chain antibody having enzyme activity in an intracellular or extracellular expression system by using an expression vector including the polynucleotide that encodes a κ light chain antibody obtained after modification.

公开(公告)号：US20160340441A1

公开(公告)日：2016-11-24

申请号：US15166647

申请日：2016-05-27

Applicant: Japan Science and Technology Agency

Inventor： Taizo UDA ,  Emi HIFUMI

IPC: C07K16/30

CPC classification number: C07K16/3023 ,  A61K2039/505 ,  C07K16/18 ,  C07K2317/21 ,  C07K2317/515 ,  C07K2317/60 ,  C07K2317/73

Abstract: According to the present invention, an anticancer agent is provided that has as an active ingredient thereof a human antibody light chain that demonstrates cytotoxicity against cancer cells and particularly lung cancer cells. The anticancer agent of the present invention primarily comprises: a human antibody κ-type light chain in the form of a dimer in which the variable region is composed of a polypeptide represented by the amino acid sequence of SEQ ID NO: 1, 9 or 13 or an amino acid sequence in which one or a plurality of amino acids have been added, deleted or substituted in these amino acid sequences; or, a human antibody κ-type light chain in the form of a monomer in which the variable region is composed of a polypeptide represented by the amino acid sequence of SEQ ID NO: 19 or an amino acid sequence in which one or a plurality of amino acids have been added, deleted or substituted in the amino acid sequence.

Abstract translation: 根据本发明，提供抗癌剂，其具有作为其活性成分的人抗体轻链，其对癌细胞特别是肺癌细胞具有细胞毒性。 本发明的抗癌剂主要包括：二抗体形式的人抗体κ型轻链，其中可变区由SEQ ID NO：1,9或13的氨基酸序列表示的多肽组成 或其中在这些氨基酸序列中添加，缺失或取代一个或多个氨基酸的氨基酸序列; 或单体形式的人抗体κ型轻链，其中可变区由SEQ ID NO：19的氨基酸序列表示的多肽或其中一个或多个 已经在氨基酸序列中添加，缺失或取代了氨基酸。