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公开(公告)号：US20190128876A1
公开(公告)日：2019-05-02
申请号：US16220040
申请日：2018-12-14
Applicant: JINAN UNIVERSITY
Inventor： Yaoxiong HUANG , Jiang He
Abstract: The presently claimed invention applies to an immunoassay apparatus based on micro-lens imaging technique and related product. In a preferred embodiment, the immunoassay apparatus comprises a monochromatic light source module, an intelligent auto-scanning and imaging module, a temperature controlled transparent toughened glass platform, a central control module, and a touchscreen displaying module. By automatically performing scan in focus imaging on the micro-lenses immersing in antigen-antibody solution and analyzing the data of the image, the apparatus can monitor the refractive index change of a sample solution before and after antigen-antibody reaction, so as to determine the concentration of antigen or antibody in the solution without requiring any labeling, expensive enzymes, pre-immobilization/modification, and post-washing. It can detect ultra-micro amount of Ag/Ab (˜pg/mL) in very low sample volume solution (several μL) within 2 minutes. It is of high accuracy and reliability. Moreover, the apparatus is simple and compact, can be portable for on-site immunoassay.
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公开(公告)号：US20190120758A1
公开(公告)日：2019-04-25
申请号：US16220020
申请日：2018-12-14
Applicant: JINAN UNIVERSITY
Inventor： Yaoxiong HUANG , Jiang He
CPC classification number: G01N21/4133 , G01N21/0303 , G01N21/253 , G01N21/431 , G01N2021/0307 , G01N2021/0382 , G01N2201/0639 , G02B13/0055
Abstract: Disclosed is a micro-lens imaging multi-well test plate which comprises: a transparent plate of 3-5 mm in thickness with one or more trapezoidal wells locating in the middle of the plate, each of the wells is of an underside of 2-4 mm in diameter, 0.2-0.5 mm in thickness, a trapezoidal dip angle of 60-75°, and has a micro-lens which upper half is hemispherical, lower half is a cylinder, with radius of 0.1˜1.0 mm, height of 0.2˜2.5 mm, molded on the bottom of the well. The micro-lens imaging multi-well test plate is made of homogeneous optical transparent materials. When the trapezoidal concave wells of the test plate are filled with fluid to immerse the micro-lens, under parallel light illumination, due to the refraction effect of light, the image of micro-lens is a round one with an outer edge that is a black ring. The outer radius R of the black ring is the radius of the micro-lens, the inner radius r of the black ring is a function of the refractive index n1 of the immersion liquid, the refractive index n2 of the micro-lens and the height h of the micro-lens, so the refractive index of the sample fluid can be determined by monitoring the value of the inner radius r of the black ring with known values of R, n2 and h. By using a multi-well test plate for imaging, the individual refractive indices of different sample fluids in all the wells can be determined simultaneously in one measurement.
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公开(公告)号：US10458910B2
公开(公告)日：2019-10-29
申请号：US16220020
申请日：2018-12-14
Applicant: JINAN UNIVERSITY
Inventor： Yaoxiong Huang , Jiang He
Abstract: Disclosed is a micro-lens imaging multi-well test plate which comprises: a transparent plate of 3-5 mm in thickness with one or more trapezoidal wells locating in the middle of the plate, each of the wells is of an underside of 2-4 mm in diameter, 0.2-0.5 mm in thickness, a trapezoidal dip angle of 60-75°, and has a micro-lens which upper half is hemispherical, lower half is a cylinder, with radius of 0.1˜1.0 mm, height of 0.2˜2.5 mm, molded on the bottom of the well. The micro-lens imaging multi-well test plate is made of homogeneous optical transparent materials. When the trapezoidal concave wells of the test plate are filled with fluid to immerse the micro-lens, under parallel light illumination, due to the refraction effect of light, the image of micro-lens is a round one with an outer edge that is a black ring. The outer radius R of the black ring is the radius of the micro-lens, the inner radius r of the black ring is a function of the refractive index n1 of the immersion liquid, the refractive index n2 of the micro-lens and the height h of the micro-lens, so the refractive index of the sample fluid can be determined by monitoring the value of the inner radius r of the black ring with known values of R, n2 and h. By using a multi-well test plate for imaging, the individual refractive indices of different sample fluids in all the wells can be determined simultaneously in one measurement.
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