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    COELENTERAZINE ANALOGS
    4.
    发明申请
    COELENTERAZINE ANALOGS 有权

    公开(公告)号：US20140302539A1

    公开(公告)日：2014-10-09

    申请号：US14244953

    申请日：2014-04-04

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO ,  Yuiko MIURA

    IPC分类号： C12Q1/66 ,  C07D487/04

    CPC分类号： C12Q1/66 ,  C07D487/04 ,  C12Q1/6897 ,  C12Y113/12007

    摘要： Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below.

    摘要翻译： 对于各种荧光素酶，期望具有与已知肠溶素类似物不同的发光性质的肠胰肽类似物。 本发明提供由下述通式（1）表示的化合物。

    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF
    7.
    发明申请
    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF 有权
    CODON优化的基因用于突变型破骨细胞及其使用方法

    公开(公告)号：US20140242574A1

    公开(公告)日：2014-08-28

    申请号：US14188838

    申请日：2014-02-25

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12N9/02 ,  C12Q1/68 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C12N9/0006 ,  C12Q1/66 ,  C12Y113/12013

    摘要： There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

    摘要翻译： 已经需要针对Oplophorus萤光素酶的突变催化结构域的密码子优化的基因，其能够在培养的动物细胞和大肠杆菌中有效地表达蛋白质。 还需要一种显示出比天然的19kDa蛋白质更高的活性的底物coelenterazine类似物。 本发明提供了包含由SEQ ID NO：2的核苷酸序列组成的多核苷酸的多核苷酸。根据本发明，双 - 肠里注胺用作底物coelenterazine类似物，其适用于由包含由核苷酸组成的多核苷酸的多核苷酸编码的光蛋白 SEQ ID NO：2的序列。

    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF
    10.
    发明申请
    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF 有权
    CODON优化的基因用于突变型破骨细胞及其使用方法

    公开(公告)号：US20170029789A1

    公开(公告)日：2017-02-02

    申请号：US15291367

    申请日：2016-10-12

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12N9/02 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C12N9/0006 ,  C12Q1/66 ,  C12Y113/12013

    摘要： There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

    摘要翻译： 已经需要针对Oplophorus萤光素酶的突变催化结构域的密码子优化的基因，其能够在培养的动物细胞和大肠杆菌中有效地表达蛋白质。 还需要一种显示出比天然的19kDa蛋白质更高的活性的底物coelenterazine类似物。 本发明提供了包含由SEQ ID NO：2的核苷酸序列组成的多核苷酸的多核苷酸。根据本发明，双 - 肠里注胺用作底物coelenterazine类似物，其适用于由包含由核苷酸组成的多核苷酸的多核苷酸编码的光蛋白 SEQ ID NO：2的序列。