利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

29 条记录 (耗时 0.022 秒)

    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    2.
    发明申请
    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF 有权
    磷脂酰胆碱酯酶催化蛋白的突变基因及其用途

    公开(公告)号：US20150184134A1

    公开(公告)日：2015-07-02

    申请号：US14576366

    申请日：2014-12-19

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12N9/02 ,  C12Q1/66

    CPC分类号： C12Q1/66 ,  C12N9/00 ,  C12N9/0069 ,  C12Y113/12005 ,  C12Y113/12007

    摘要： Secreted luciferases which are different from those known heretofore have been desired. The present invention provides a luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from amino acids at the positions of 1 to 4 is deleted in the amino acid sequence of SEQ ID NO: 2.

    摘要翻译： 已经期望与之前已知的分泌的萤光素酶不同。 本发明提供了包含氨基酸序列的荧光素酶突变体，其中在SEQ ID NO：2的氨基酸序列中缺失选自1至4位的氨基酸的至少一个氨基酸。

    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF
    3.
    发明申请
    CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF 有权
    CODON优化的基因用于突变型破骨细胞及其使用方法

    公开(公告)号：US20160298171A1

    公开(公告)日：2016-10-13

    申请号：US15191730

    申请日：2016-06-24

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12Q1/66 ,  C12N9/02

    CPC分类号： C12N9/0069 ,  C12N9/0006 ,  C12Q1/66 ,  C12Y113/12013

    摘要： There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analogue showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analogue suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.

    摘要翻译： 已经需要针对Oplophorus萤光素酶的突变催化结构域的密码子优化的基因，其能够在培养的动物细胞和大肠杆菌中有效地表达蛋白质。 还需要一种显示出比天然的19kDa蛋白质更高的活性的底物coelenterazine类似物。 本发明提供了包含由SEQ ID NO：2的核苷酸序列组成的多核苷酸的多核苷酸。根据本发明，双 - 肠里注胺用作底物coelenterazine类似物，其适用于由包含由核苷酸组成的多核苷酸的多核苷酸编码的光蛋白 SEQ ID NO：2的序列。

    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    4.
    发明申请
    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF 有权
    磷脂酰胆碱酯酶催化蛋白的突变基因及其用途

    公开(公告)号：US20150299675A1

    公开(公告)日：2015-10-22

    申请号：US14685643

    申请日：2015-04-14

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Yuiko MIURA ,  Junichi SATO

    IPC分类号： C12N9/02 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C12Y113/12 ,  C12Y113/12013

    摘要： A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.

    摘要翻译： 已经需要与常规荧光素酶不同的新型荧光素酶。 在SEQ ID NO：2的氨基酸序列中，包含其中4位谷氨酸，11位精氨酸，18位亮氨酸和27位缬氨酸被氨基酸序列取代的氨基酸序列的荧光素酶突变体。

    CHIMERIC GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    5.
    发明申请
    CHIMERIC GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF 审中-公开
    磷脂酰胆碱酯酶催化蛋白的重组基因及其用途

    公开(公告)号：US20150259652A1

    公开(公告)日：2015-09-17

    申请号：US14641908

    申请日：2015-03-09

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12N9/02 ,  G01N33/573

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C12Y113/12013 ,  G01N2333/90241

    摘要： A novel luciferase that is distinct from conventional luciferase has been desired.A luciferase mutant comprising an amino acid sequence in which tyrosine at position of 138 is substituted with another amino acid and at least 3 amino acids selected from the group consisting of isoleucine at position of 90, proline at position of 115, glutamine at position of 124 and asparagine at position of 166 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.

    摘要翻译： 已经需要与常规荧光素酶不同的新型荧光素酶。 一种荧光素酶突变体，其包含其中138位的酪氨酸被另一个氨基酸取代的氨基酸序列，以及选自位于90的异亮氨酸，115位的脯氨酸和124位的谷氨酰胺的至少3个氨基酸 在166位的天冬酰胺被SEQ ID NO：2的氨基酸序列中的其它氨基酸取代。

    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF
    6.
    发明申请
    MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF 有权
    磷脂酰胆碱酯酶催化蛋白的突变基因及其用途

    公开(公告)号：US20150111233A1

    公开(公告)日：2015-04-23

    申请号：US14516666

    申请日：2014-10-17

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Junichi SATO

    IPC分类号： C12N9/02 ,  C12Q1/66

    CPC分类号： C12N9/0069 ,  C07K14/43509 ,  C12Q1/66 ,  C12Y113/12007

    摘要： Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2.

    摘要翻译： 与以前已知的荧光素酶不同的荧光素酶是期望的。 一种荧光素酶突变体，其包含氨基酸序列，其中至少一个选自44位的缬氨酸，54位的丙氨酸和138位的酪氨酸的氨基酸被其它氨基酸取代， 在SEQ ID NO：2的氨基酸序列中。

    PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM
    7.
    发明申请
    PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM 有权
    生产重组蛋白作为可溶形式的方法

    公开(公告)号：US20150087047A1

    公开(公告)日：2015-03-26

    申请号：US14476624

    申请日：2014-09-03

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Yuiko SAHARA

    IPC分类号： C07K14/31 ,  C12N9/02

    CPC分类号： C07K14/31 ,  C07K2319/61 ,  C07K2319/705 ,  C12N9/0069 ,  C12N15/62 ,  C12Y113/12005 ,  C12Y113/12007 ,  C12Y113/12013

    摘要： A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.

    摘要翻译： 使用重组蛋白表达系统制备靶蛋白作为可溶性蛋白质。 使用表达载体，其包括（1）表达诱导型启动子序列; （2）包含编码由式（Z）n表示的多肽的多核苷酸的第一编码序列; 和（3）包含编码靶蛋白的多核苷酸的第二编码序列。 还使用包含使用该表达载体表达蛋白质的靶蛋白质的制造方法。