公开(公告)号：US20220403369A1

公开(公告)日：2022-12-22

申请号：US17775626

申请日：2020-07-02

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolievich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignaty Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Julia Valerevna ANDREEVA ,  Tatiana Igorevna ZYUBKO ,  Iana Vitalevna FEDOROVA ,  Mikhail Alekseevich KHODORKOVSKII ,  George Evgenevich POBEGALOV ,  Anatoliy Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILIEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N15/10 ,  C07K14/00 ,  C12Q1/6806

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for modifying the genomic DNA sequence in the cell of a unicellular organism or a multicellular organism. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which fact will enable the use of various variants of Cas9 nucleases for cutting genomic or plasmid DNA in various organisms, in a larger number of specific sites and/or under various conditions.

公开(公告)号：US20220228134A1

公开(公告)日：2022-07-21

申请号：US17617039

申请日：2020-06-30

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolievich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignaty Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Julia Valerevna ANDREEVA ,  Tatiana Igorevna ZYUBKO ,  Yana Vitalievna FEDOROVA ,  Mikhail Alekseevich KHODORKOVSKII ,  George Evgenevich POBEGALOV ,  Anatoliy Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILIEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N9/22 ,  C07K14/00

Abstract: The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. The present nuclease possesses unusual properties and can be used as an instrument for introducing changes at strictly specified locations in a genomic DNA sequence of single-celled and multi-celled organisms. The invention thus increases the universality of accessible CRISPR-Cas9 systems, making it possible to use Cas9 nuclease from various organisms to cut genomic or plasmid DNA in a large number of specific sites and under various conditions.