公开(公告)号：US20220017896A1

公开(公告)日：2022-01-20

申请号：US17296629

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N15/11 ,  C12N9/22

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Defluviimonas sp. 20V17, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and specific conditions.

公开(公告)号：US20220403369A1

公开(公告)日：2022-12-22

申请号：US17775626

申请日：2020-07-02

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolievich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignaty Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Julia Valerevna ANDREEVA ,  Tatiana Igorevna ZYUBKO ,  Iana Vitalevna FEDOROVA ,  Mikhail Alekseevich KHODORKOVSKII ,  George Evgenevich POBEGALOV ,  Anatoliy Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILIEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N15/10 ,  C07K14/00 ,  C12Q1/6806

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for modifying the genomic DNA sequence in the cell of a unicellular organism or a multicellular organism. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which fact will enable the use of various variants of Cas9 nucleases for cutting genomic or plasmid DNA in various organisms, in a larger number of specific sites and/or under various conditions.

公开(公告)号：US20220228134A1

公开(公告)日：2022-07-21

申请号：US17617039

申请日：2020-06-30

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolievich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignaty Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Julia Valerevna ANDREEVA ,  Tatiana Igorevna ZYUBKO ,  Yana Vitalievna FEDOROVA ,  Mikhail Alekseevich KHODORKOVSKII ,  George Evgenevich POBEGALOV ,  Anatoliy Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILIEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N9/22 ,  C07K14/00

Abstract: The present invention describes a novel bacterial nuclease of a CRISPR-Cas9 system from the bacterium P. pneumotropica, as well as the use of said nuclease for creating strictly specific two-strand cuts in a DNA molecule. The present nuclease possesses unusual properties and can be used as an instrument for introducing changes at strictly specified locations in a genomic DNA sequence of single-celled and multi-celled organisms. The invention thus increases the universality of accessible CRISPR-Cas9 systems, making it possible to use Cas9 nuclease from various organisms to cut genomic or plasmid DNA in a large number of specific sites and under various conditions.

公开(公告)号：US20220064612A1

公开(公告)日：2022-03-03

申请号：US17276016

申请日：2019-09-13

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Dmitriy Aleksandrovich MADERA ,  Aleksandr Vladimirovich KARABELSKII ,  Roman Alekseevich IVANOV ,  Dmitry Valentinovich MOROZOV ,  Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Dmitrii Aleksandrovich SUTORMIN ,  Georgii Evgenevich POBEGALOV ,  Aleksandra Andreevna VASILEVA ,  Polina Anatolevna SELKOVA ,  Anatolii Nikolaevich ARSENIEV ,  Tatyana Igorevna ZYUBKO ,  Iana Vitalevna FEDOROVA

IPC: C12N9/22 ,  C12N15/79

Abstract: The present invention relates to the field of biotechnology, molecular biology and medicine, in particular to nuclease enzyme and use thereof. More specifically, the present invention relates to PaCas9 nuclease enzyme. The invention also relates to a nucleic acid encoding said nuclease, a genetic construct, an expression vector, a delivery vector, which comprise said nucleic acid, a liposome comprising said nuclease or nucleic acid encoding said nuclease, a method for producing a nuclease, methods for delivery, and a host cell comprising a nucleic acid encoding said nuclease.

公开(公告)号：US20220002692A1

公开(公告)日：2022-01-06

申请号：US17296597

申请日：2019-11-26

Applicant: JOINT STOCK COMPANY "BIOCAD"

Inventor： Konstantin Viktorovich SEVERINOV ,  Sergey Anatolevich SHMAKOV ,  Daria Nikolaevna ARTAMONOVA ,  Ignatiy Igorevich GORYANIN ,  Olga Sergeevna MUSHAROVA ,  Iuliia Valerevna PISKUNOVA ,  Iana Vitalevna FEDOROVA ,  Tatyana Igorevna ZYUBKO ,  Mikhail Alekseevich KHODORKOVSKIY ,  Georgii Evgenevich POBEGALOV ,  Anatolii Nikolaevich ARSENIEV ,  Polina Anatolevna SELKOVA ,  Aleksandra Andreevna VASILEVA ,  Tatiana Olegovna ARTAMONOVA ,  Marina Viktorovna ABRAMOVA

IPC: C12N9/22 ,  C12N15/90 ,  C12N15/11

Abstract: The present invention describes a novel bacterial nuclease of the CRISPR-Cas9 system from the bacterium Clostridium celluloliticum, as well as the use thereof to form strictly specific double-strand breaks in a DNA molecule. This nuclease has unusual properties and may be used as a tool for introducing modifications at strictly defined sites in the genomic DNA sequence of unicellular or multicellular organisms. Thus, the versatility of the available CRISPR-Cas9 systems is increased, which will enable the use of Cas9 nucleases from various organisms for cutting genomic or plasmid DNA in a larger number of specific sites and in wider temperature ranges. Further, provided is more facile editing of the genome of the biotechnologically significant bacterium Clostridium celluloliticum.