公开(公告)号：US12173336B2

公开(公告)日：2024-12-24

申请号：US17276016

申请日：2019-09-13

Applicant: JOINT STOCK COMPANY “BIOCAD”

Inventor： Dmitriy Aleksandrovich Madera ,  Aleksandr Vladimirovich Karabelskii ,  Roman Alekseevich Ivanov ,  Dmitry Valentinovich Morozov ,  Konstantin Viktorovich Severinov ,  Sergey Anatolevich Shmakov ,  Dmitrii Aleksandrovich Sutormin ,  Georgii Evgenevich Pobegalov ,  Aleksandra Andreevna Vasileva ,  Polina Anatolevna Selkova ,  Anatolii Nikolaevich Arseniev ,  Tatyana Igorevna Zyubko ,  Iana Vitalevna Fedorova

IPC: C12N9/22 ,  C12N15/79

Abstract: The present invention relates to the field of biotechnology, molecular biology and medicine, in particular to nuclease enzyme and use thereof. More specifically, the present invention relates to PaCas9 nuclease enzyme. The invention also relates to a nucleic acid encoding the nuclease, a genetic construct, an expression vector, a delivery vector, which comprise the nucleic acid, a liposome comprising the nuclease or nucleic acid encoding the nuclease, a method for producing a nuclease, methods for delivery, and a host cell comprising a nucleic acid encoding the nuclease.