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1.发明授权公开(公告)号：US06881537B1
公开(公告)日：2005-04-19
申请号：US09463352
申请日：1998-08-05
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV).With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants.The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
摘要翻译： 本发明涉及可用于病毒诊断领域的核酸序列，更具体地说涉及导致人免疫缺陷病毒（HIV）的AIDS感染的诊断。 本发明提供了可用作扩增和检测HIV-1核酸的引物和探针的核苷酸序列。 本发明提供的寡核苷酸序列位于HIV病毒基因组的LTR部分。 已经发现，通过将本发明的序列用于扩增和检测核酸的方法，可以获得HIV-1的敏感和特异性检测。 本发明的序列的益处主要在于以下事实：借助包含本发明序列的引物和探针，可以高精度和灵敏度检测所有目前已知的HIV-1亚型的核酸 。 迄今为止，还没有开发可以检测到这样广泛的HIV-1变体的引物对或杂交探针。 根据本发明的寡核苷酸序列特别可用于扩增核酸的方法。
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2.发明授权公开(公告)号：US08697352B2
公开(公告)日：2014-04-15
申请号：US11108233
申请日：2005-04-18
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
摘要翻译： 本发明涉及可用于病毒诊断领域的核酸序列，更具体地说涉及导致人免疫缺陷病毒（HIV）的AIDS感染的诊断。 本发明提供了可用作扩增和检测HIV-1核酸的引物和探针的核苷酸序列。 本发明提供的寡核苷酸序列位于HIV病毒基因组的LTR部分。 已经发现，通过将本发明的序列用于扩增和检测核酸的方法，可以获得HIV-1的敏感和特异性检测。 本发明的序列的益处主要在于以下事实：借助包含本发明序列的引物和探针，可以高精度和灵敏度检测所有目前已知的HIV-1亚型的核酸 。 迄今为止，还没有开发可以检测到这样广泛的HIV-1变体的引物对或杂交探针。 根据本发明的寡核苷酸序列特别可用于扩增核酸的方法。
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3.发明申请公开(公告)号：US20060223056A1
公开(公告)日：2006-10-05
申请号：US11372577
申请日：2006-03-10
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
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公开(公告)号：US20050239118A1
公开(公告)日：2005-10-27
申请号：US11108233
申请日：2005-04-18
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
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5.发明申请公开(公告)号：US20060223057A1
公开(公告)日：2006-10-05
申请号：US11372586
申请日：2006-03-10
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
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6.发明申请公开(公告)号：US20060228699A1
公开(公告)日：2006-10-12
申请号：US11372585
申请日：2006-03-10
CPC分类号： C12Q1/703 , C12Q1/6848 , Y10S435/81
摘要： The present invention is related to nucleic acid sequences that can be used in the field of virus diagnostics, more specifically the diagnosis of infections with the AIDS causing Human Immuno-deficiency Virus (HIV). With the present invention nucleotide sequences are provided that can be used as primers and probes in the amplification and detection of HIV-1 nucleic acid. The oligonucleotide sequences provided with the present invention are located in the LTR part of the HIV viral genome. It has been found that, by using the sequences of the present invention in methods for the amplification and detection of nucleic acid a sensitive and specific detection of HIV-1 can be obtained. The benefit of the sequences of the present invention primarily resides in the fact that, with the aid of primers and probes comprising the sequences according to the invention the nucleic acid of all presently known subtypes of HIV-1 can be detected with high accuracy and sensitivity. So far no primer pairs or hybridization probes have been developed that would allow the detection of such a broad range of HIV-1 variants. The oligonucleotide sequences according to the present invention are especially useful in methods for the amplification of nucleic acid.
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公开(公告)号：US06489110B1
公开(公告)日：2002-12-03
申请号：US09600770
申请日：2000-07-21
申请人： Pieter Oudshoorn , Paul R. Klatser
发明人： Pieter Oudshoorn , Paul R. Klatser
IPC分类号： C12Q168
CPC分类号： C12Q1/689
摘要： The present invention is related to the detection of bacteria, such as Mycobacteria, in human or animal body fluids such as blood, sputum and urine. The present invention provides a method for assessing the viability of bacteria such as Mycobacterium tuberculosis without the need for propagation of the bacteria. The method of the present invention is in particular useful for assessing the viability of Mycobacteria species such as are M. tuberculosis or M. leprae. With the present invention oligonucleotides are provided that can be used as primers and probes for the amplification of bacterial EF-Tu mRNA. The use of the oligonucleotides according to the invention is not limited to any particular amplification technique or any particular modification thereof. It is evident that the oligonucleotides according to the invention find their use in many different nucleic aid amplification techniques and various methods for detecting the presence of (amplified) bacterial EF-Tu mRNA.
摘要翻译： 本发明涉及在人体或动物体液如血液，痰液和尿液中检测细菌如分枝杆菌。 本发明提供了一种用于评估细菌如结核分枝杆菌的活力的方法，而不需要繁殖细菌。 本发明的方法特别可用于评估分枝杆菌属物种如M.tuberculosis或M. leprae的活力。 本发明提供了可用作扩增细菌EF-Tu mRNA的引物和探针的寡核苷酸。 根据本发明的寡核苷酸的使用不限于任何特定的扩增技术或其任何具体的修饰。 很明显，根据本发明的寡核苷酸可用于许多不同的核酸扩增技术和用于检测（扩增的）细菌EF-Tu mRNA的存在的各种方法。
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