公开(公告)号：US07132288B2

公开(公告)日：2006-11-07

申请号：US10467982

申请日：2002-02-14

申请人： Jakob Izbicki ,  Stefan Hosch ,  Peter Scheunemann

发明人： Jakob Izbicki ,  Stefan Hosch ,  Peter Scheunemann

IPC分类号： C12N5/00

CPC分类号： C12N5/0693 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2501/11 ,  C12N2501/148

摘要： Disclosed is a medium for cultivating tumor cells, comprising the following constituents, as comprised for example in the medium RPMI 1640: (a) fetal calf serum (FCS), (b) penicillin-streptomycin, (c) L-glutamine, (d) transferrin, (e) insulin, (f) human epidermal growth factor (EGF) and (g) α-TGF. A method for characterizing tumor cells or potential tumor cells, respectively, is also described. Said method includes the culturing of cells in the inventive medium and the two tumor cell lines LNHOS1 and PTHOS1 established by means of the inventive culturing method.

摘要翻译： 公开了用于培养肿瘤细胞的培养基，其包含例如在培养基RPMI 1640中包含的以下成分：（a）胎牛血清（FCS），（b）青霉素 - 链霉素，（c）L-谷氨酰胺，（d ）转铁蛋白，（e）胰岛素，（f）人表皮生长因子（EGF）和（g）α-TGF。 还描述了分别表征肿瘤细胞或潜在肿瘤细胞的方法。 所述方法包括培养本发明培养基中的细胞和通过本发明培养方法建立的两个肿瘤细胞系LNHOS1和PTHOS1。