公开(公告)号：US20240360412A1

公开(公告)日：2024-10-31

申请号：US18294221

申请日：2022-08-04

申请人： ANYADI, LLC

发明人： Rachit OHRI ,  Donna SONNTAG ,  Jason CAHOON ,  Graeme LAMBERT

IPC分类号： C12N5/0783

CPC分类号： C12N5/0646 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/76 ,  C12N2501/105 ,  C12N2501/33 ,  C12N2501/599

摘要： The invention of the current disclosure provides cell culture medium formulations useful for the culture of Natural Killer (NK) immune cells. Also included are methods of culture, cryopreservation, and transduction of NK cells comprising said culture medium formulations.

公开(公告)号：US12129477B2

公开(公告)日：2024-10-29

申请号：US17578425

申请日：2022-01-18

申请人： Juno Therapeutics GmbH

发明人： Keenan Bashour ,  Semih U. Tareen

IPC分类号： C12N15/86 ,  C07K14/315 ,  C07K14/725 ,  C07K16/28 ,  C12N5/0783

CPC分类号： C12N15/86 ,  C07K14/315 ,  C07K14/7051 ,  C07K16/2809 ,  C07K16/2818 ,  C12N5/0636 ,  C07K2317/55 ,  C07K2319/00 ,  C12N2500/32 ,  C12N2501/515 ,  C12N2740/10043

摘要： Provided herein are methods for transducing a plurality of cells in a composition of cells, such as a population of lymphocytes, containing viral particles. In some aspects, provided methods and reagents for the transduction of cell populations involve binding of agents to a molecule on the surface of the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for transduction and/or expansion or proliferation or other stimulation of a population of cells, and then such agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.

公开(公告)号：US20240316115A1

公开(公告)日：2024-09-26

申请号：US18579093

申请日：2022-07-14

申请人： Astellas Pharma Inc. ,  OSAKA UNIVERSITY

发明人： Kenichiro SHIMATANI ,  Hiromu SATO ,  Masao SASAI ,  Yoshiki SAWA ,  Atsuhiro SAITO ,  Shigeru MIYAGAWA

IPC分类号： A61K35/44 ,  C12N5/071

CPC分类号： A61K35/44 ,  C12N5/069 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2506/02 ,  C12N2509/00 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54

摘要： An object of the present invention is to provide a pericyte-like cell having high angiogenic potential with a higher cell proliferation ability than a primary pericyte available in the past and high VEGF expression, and a method for producing the same. Provided are a method for producing a VEGF-highly expressing pericyte-like cell, the method including selecting a CD56(−) pericyte-like cell from a population including a pericyte-like cell obtained by inducing differentiation of a pluripotent stem cell; and a VEGF-highly expressing pericyte-like cell produced by the production method.

公开(公告)号：US12091683B2

公开(公告)日：2024-09-17

申请号：US16648540

申请日：2018-09-19

申请人： MEGAKARYON CORPORATION

发明人： Haruki Okamoto ,  Chisato Tokikura ,  Tomohiro Shigemori

IPC分类号： C12N5/078

CPC分类号： C12N5/0644 ,  C12N2500/32 ,  C12N2506/45

摘要： The present invention provides a method for producing platelets that can improve at least one of the ability of megakaryocyte to produce platelets and the bioactivity of platelets produced even in high-density culture, for example. The method for producing platelets of the present invention includes a platelet producing step of producing platelets from megakaryocytes, wherein the platelet producing step is performed in the presence of at least one of glycine and cysteine.

公开(公告)号：US20240279604A1

公开(公告)日：2024-08-22

申请号：US18582703

申请日：2024-02-21

申请人： Industry-University Cooperation Foundation Hanyang University Erica Campus

发明人： Chong Suk RYOU ,  Hyun Jung PARK

IPC分类号： C12N5/0793 ,  C12M1/00 ,  C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0619 ,  C12M23/02 ,  C12N5/0018 ,  C12N5/0622 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2501/115 ,  C12N2503/02 ,  C12N2506/08 ,  C12N2513/00

摘要： The present invention relates to a method for producing a spheroid including neurons and astrocytes, the method including: culturing induced pluripotent stem cell-neural progenitor cells (iPSC-NPCs) in an NPC medium; culturing the cultured cells in a neuronal differentiation medium (NDM); and culturing the cultured cells in a glial differentiation medium (GDM). The spheroid produced by the method according to the present invention contains mature neurons and astrocytes which express a desired marker after 3 weeks of in vitro culture and has a uniform size, and thus can be used for simple visual screening to confirm drug efficacy.

公开(公告)号：US20240245832A1

公开(公告)日：2024-07-25

申请号：US18551085

申请日：2022-03-18

申请人： THE UNIVERSITY OF SYDNEY ,  SOUTH EASTERN SYDNEY LOCAL HEALTH DISTRICT

发明人： Jingjing YOU ,  Gerard SUTTON ,  Morgan OVERMASS ,  Constantinos PETSOGLOU ,  Sheng HUA ,  Li WEN

IPC分类号： A61L27/38 ,  A61L27/24 ,  A61L27/36 ,  A61L27/54 ,  C12N5/079

CPC分类号： A61L27/3808 ,  A61L27/24 ,  A61L27/3616 ,  A61L27/3687 ,  A61L27/54 ,  C12N5/0621 ,  A61L2300/414 ,  A61L2430/16 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/165 ,  C12N2533/54 ,  C12N2533/56 ,  C12N2537/10

摘要： The present invention relates to bioengineered collagen constructs and methods for the preparation and use of said constructs. The constructs and methods may find application in, but are not limited to, the provision of replacement tissue and/or cells, and/or the delivery of agents to biological targets such as tissues and cells. The constructs and methods may also find application in, but are not limited to, replacing endothelial tissue.

公开(公告)号：US12043846B2

公开(公告)日：2024-07-23

申请号：US16899121

申请日：2020-06-11

申请人： Ncardia B.V.

发明人： Stefan Robbert Braam

IPC分类号： C12N5/077

CPC分类号： C12N5/0657 ,  C12N2500/25 ,  C12N2500/30 ,  C12N2500/32 ,  C12N2500/33 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/30 ,  C12N2501/33 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/998 ,  C12N2506/02 ,  C12N2506/45

摘要： The current disclosure relates to a culture medium, different methods to generate adult-like cardiomyocytes from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC) using the medium, in particular from stem cells that differentiated into (foetal) cardiomyocytes, and to kits comprising the medium, or the medium together with differentiated (foetal) cardiomyocytes derived from pluripotent embryonic stem cells (ESC) and/or (induced) pluripotent stem cells (iPSC).

公开(公告)号：US12043845B2

公开(公告)日：2024-07-23

申请号：US16620091

申请日：2018-06-08

申请人： Polpharma Biologics S.A.

发明人： Louis Boon ,  Lenneke De Winter ,  Tomasz Sitar

IPC分类号： C12N5/00 ,  C12N15/67

CPC分类号： C12N5/0018 ,  C12N15/67 ,  C12N2500/32 ,  C12N2500/34

摘要： The invention provide a method for reducing the amount of acidic species and/or increasing the amount of basic species in a composition comprising a recombinant protein or for increasing the specific production rate of mammalian cells expressing a recombinant protein to simplify subsequent clarification and purification of the recombinant protein, said method comprising culturing mammalian cells expressing said recombinant protein in a cell culture medium comprising citrulline.

公开(公告)号：US12010963B2

公开(公告)日：2024-06-18

申请号：US17720652

申请日：2022-04-14

申请人： SHANDONG DAFENGYUAN AGRICULTURE CO., LTD.

发明人： Yangyan Zhou ,  Weijian Sun ,  Wenxiu Chen ,  Aowei Mo ,  Peng Guo ,  Penghao Xu

IPC分类号： A01H6/36 ,  A01G22/05 ,  A01G24/15 ,  A01G24/22 ,  A01H4/00 ,  A01H5/08 ,  C12N5/00 ,  C12N5/04

CPC分类号： A01H4/002 ,  A01G22/05 ,  A01G24/15 ,  A01G24/22 ,  A01H4/001 ,  A01H5/08 ,  A01H6/368 ,  C12N5/0025 ,  C12N5/04 ,  C12N2500/05 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/60 ,  C12N2501/30 ,  C12N2533/76

摘要： The invention provides a callus induction medium for blueberry tissue culture, taking woody plant medium (WPM) as a basic medium, and including: 0.5-5.0 mg/L forchlorfenuron (CPPU) and 0.1-0.4 mg/L 2-isopentenyladenine (2-ip). The present invention also provides a callus culture method for blueberry, including inoculating the blueberry explant into the above callus induction medium to conduct induction culture in order to form blueberry callus. The present invention also discloses the medium combination and culture method to culture the above blueberry callus to blueberry tissue culture plant. For the above medium and culture method, the differentiation effect is good, efficiency is high, one can conduct continuous differentiation, and the effect is better on multiple varieties.

公开(公告)号：US20240182854A1

公开(公告)日：2024-06-06

申请号：US18450234

申请日：2023-08-15

申请人： The Trustees of the University of Pennsylvania ,  Regents of the University of Minnesota

发明人： James L. Riley ,  Carl H. June ,  Bruce R. Blazar ,  Keli Hippen

IPC分类号： C12N5/0783

CPC分类号： C12N5/0637 ,  C12N2500/32 ,  C12N2501/04 ,  C12N2501/2302 ,  C12N2501/51 ,  C12N2501/515

摘要： The present invention provides compositions and methods for expanding natural T regulatory cells (nTregs) without substantially sacrificing suppressive function of the cells. Accordingly, the invention provides uses of the expanded nTregs for cellular therapy.