摘要:
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
摘要翻译:提供了一种预测蛋白质中的内含肽插入位点将导致切换表型的方法。 该方法包括鉴定蛋白内的多个C / T / S位点; 从多个C / T / S /位点中选择由支持向量机排列为0.75或更高的那些,在蛋白质的活性位点的10埃内,以及在环 - 环α螺旋结。 还提供了控制蛋白质活性的方法和包含具有受控活性的蛋白质的宿主。 此外,提供了内含肽修饰的蛋白质和含有内含肽修饰的蛋白质的植物。
摘要:
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
摘要翻译:提供了一种预测蛋白质中的内含肽插入位点将导致切换表型的方法。 该方法包括鉴定蛋白内的多个C / T / S位点; 从多个C / T / S /位点中选择由支持向量机排列为0.75或更高的那些,在蛋白质的活性位点的10埃内,以及在环 - 环α螺旋结。 还提供了控制蛋白质活性的方法和包含具有受控活性的蛋白质的宿主。 此外,提供了内含肽修饰的蛋白质和含有内含肽修饰的蛋白质的植物。
摘要:
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
摘要翻译:提供了一种预测蛋白质中的内含肽插入位点将导致切换表型的方法。 该方法包括鉴定蛋白内的多个C / T / S位点; 从多个C / T / S /位点中选择由支持向量机排列为0.75或更高的那些,在蛋白质的活性位点的10埃内,以及在环 - 环α螺旋结。 还提供了控制蛋白质活性的方法和包含具有受控活性的蛋白质的宿主。 此外,提供了内含肽修饰的蛋白质和含有内含肽修饰的蛋白质的植物。
摘要:
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
摘要翻译:提供了一种预测蛋白质中的内含肽插入位点将导致切换表型的方法。 该方法包括鉴定蛋白内的多个C / T / S位点; 从多个C / T / S /位点中选择由支持向量机排列为0.75或更高的那些,在蛋白质的活性位点的10埃内,以及在环-β-片结或环 -α-螺旋结。 还提供了控制蛋白质活性的方法和包含具有受控活性的蛋白质的宿主。 此外,提供了内含肽修饰的蛋白质和含有内含肽修饰的蛋白质的植物。
摘要:
A process for the maintenance and proliferation of defective, non-infectious viruses or virus genomes, comprising mutations, i.e. deletions, substitutions, insertions and new rearrangements of the viral genes or combinations of the virus DNA with heterologous genetic material in proliferating plant material and for the regeneration of whole plants containing stably integrated into their genome said defective, non-infectious virus DNA.