公开(公告)号：US20060021087A1

公开(公告)日：2006-01-26

申请号：US11102026

申请日：2005-04-08

申请人： James Baum ,  Larry Gilbertson ,  David Kovalic ,  Thomas LaRosa ,  Maolong Lu ,  Tichafa Munyikwa ,  James Roberts ,  Wei Wu ,  Bei Zhang

发明人： James Baum ,  Larry Gilbertson ,  David Kovalic ,  Thomas LaRosa ,  Maolong Lu ,  Tichafa Munyikwa ,  James Roberts ,  Wei Wu ,  Bei Zhang

IPC分类号： A01H1/00 ,  C12N5/04 ,  C07H21/04 ,  C12N15/82

CPC分类号： C12N15/8286 ,  Y02A40/162

摘要： The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US20070259785A1

公开(公告)日：2007-11-08

申请号：US11674005

申请日：2007-02-12

申请人： Gregory Heck ,  Tichafa Munyikwa ,  Jean Goley ,  James Roberts ,  Scott Johnson ,  Ty Vaughn

发明人： Gregory Heck ,  Tichafa Munyikwa ,  Jean Goley ,  James Roberts ,  Scott Johnson ,  Ty Vaughn

IPC分类号： A01N25/26 ,  A01H5/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/82 ,  C12N5/04 ,  C12Q1/68

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N15/8218 ,  C12N15/8286 ,  C12N2310/14 ,  C12N2320/10 ,  Y02A40/162

摘要： The invention provides methods for selecting nucleotide sequences that yield dsRNA-mediated gene suppression in a target organism and enable their uptake by the target organism. The invention further provides expression constructs that confer stabilized expression of such sequences in a transgenic host cell, and methods for their use. Also provided are organisms, cells and tissues prepared by a method of the invention.

摘要翻译： 本发明提供了选择在目标生物体中产生dsRNA介导的基因抑制并使其能够被靶生物吸收的核苷酸序列的方法。 本发明进一步提供赋予转基因宿主细胞中此类序列稳定表达的表达构建体及其使用方法。 还提供了通过本发明的方法制备的生物，细胞和组织。