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公开(公告)号：US09534224B2
公开(公告)日：2017-01-03
申请号：US10535128
申请日：2003-11-14
CPC分类号： C12N15/67 , C12N15/11 , C12N2310/53 , C12Q1/6897
摘要： The present invention provides nucleic acid molecules, DNA constructs, plasmids, and methods for post-transcriptional regulation of gene expression using RNA molecules to both repress and activate translation of an open reading frame. Repression of gene expression is achieved through the presence of a regulatory nucleic acid element (the cis-repressive RNA or crRNA) within the 5′ untranslated region (5′ UTR) of an mRNA molecule. The nucleic acid element forms a hairpin (stem/loop) structure through complementary base pairing. The hairpin blocks access to the mRNA transcript by the ribosome, thereby preventing translation. In particular, in embodiments of the invention designed to operate in prokaryotic cells, the stem of the hairpin secondary structure sequesters the ribosome binding site (RBS). In embodiments of the invention designed to operate in eukaryotic cells, the stem of the hairpin is positioned upstream of the start codon, anywhere within the 5′ UTR of an mRNA. A small RNA (trans-activating RNA, or taRNA), expressed in trans, interacts with the crRNA and alters the hairpin structure. This alteration allows the ribosome to gain access to the region of the transcript upstream of the start codon, thereby activating transcription from its previously repressed state.
摘要翻译： 本发明提供核酸分子，DNA构建体，质粒和用于转录后调节基因表达的方法，其使用RNA分子抑制和激活开放阅读框的翻译。 通过在mRNA分子的5'非翻译区（5'UTR）内存在调节性核酸元件（顺式抑制性RNA或crRNA）来实现基因表达的抑制。 核酸元件通过互补碱基配对形成发夹（茎/环）结构。 发夹阻止核糖体进入mRNA转录物，从而阻止翻译。 特别地，在设计用于在原核细胞中操作的本发明的实施方案中，发夹二级结构的茎螯合核糖体结合位点（RBS）。 在设计用于在真核细胞中操作的本发明的实施方案中，发夹的茎位于起始密码子的上游，位于mRNA的5'UTR内的任何位置。 以反式表达的小RNA（反式激活RNA或taRNA）与crRNA相互作用并改变发夹结构。 这种改变允许核糖体进入起始密码子上游的转录物区域，从而从其先前的抑制状态激活转录。
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公开(公告)号：US08366371B2
公开(公告)日：2013-02-05
申请号：US11948131
申请日：2007-11-30
IPC分类号： B65G1/00
CPC分类号： H01M2/1005 , B65G1/0407 , H01M2/1077
摘要： A battery movement, storage, and charging system for large size batteries used in lift trucks includes spaced, parallel storage racks, each of which have multiple storage and charging bays. A battery transport tower is positioned in an aisle between the storage racks and is moveable horizontally between the two rows of racks. The tower includes an extendable fork lift mounted which may rotate around the axis of the tower as well as move vertically upwardly or downwardly on the tower. A battery exchanger loads a discharged battery from a lift truck, places it on a pallet and then transfers the pallet and battery to a transfer bay in a storage rack where the tower forklift moves a pallet and battery to a selected charging bay.
摘要翻译： 用于起重车的大型电池的电池移动，存储和充电系统包括间隔开的并联存储架，每个存储架具有多个存储和充电槽。 电池运输塔位于存储架之间的通道中，并且可在两排机架之间水平移动。 塔架包括一个可伸缩的叉车，其可围绕塔架的轴线旋转，并且在塔架上垂直向上或向下移动。 电池交换机从起重机装载放电的电池，将其放在托盘上，然后将托盘和电池转移到存储架中的托架上，塔式叉车将托盘和电池移动到选定的充电槽。
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公开(公告)号：US20090139940A1
公开(公告)日：2009-06-04
申请号：US11948131
申请日：2007-11-30
CPC分类号： H01M2/1005 , B65G1/0407 , H01M2/1077
摘要： A battery movement, storage, and charging system for large size batteries used in lift trucks includes spaced, parallel storage racks, each of which have multiple storage and charging bays. A battery transport tower is positioned in an aisle between the storage racks and is moveable horizontally between the two rows of racks. The tower includes an extendable fork lift mounted which may rotate around the axis of the tower as well as move vertically upwardly or downwardly on the tower. A battery exchanger loads a discharged battery from a lift truck, places it on a pallet and then transfers the pallet and battery to a transfer bay in a storage rack where the tower forklift moves a pallet and battery to a selected charging bay.
摘要翻译： 用于起重车的大型电池的电池移动，存储和充电系统包括间隔开的并联存储架，每个存储架具有多个存储和充电槽。 电池运输塔位于存储架之间的通道中，并且可在两排机架之间水平移动。 塔架包括一个可伸缩的叉车，其可围绕塔架的轴线旋转，并且在塔架上垂直向上或向下移动。 电池交换机从起重机装载放电的电池，将其放在托盘上，然后将托盘和电池转移到存储架中的托架上，塔式叉车将托盘和电池移动到选定的充电槽。
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公开(公告)号：US06773898B1
公开(公告)日：2004-08-10
申请号：US10238436
申请日：2002-09-09
IPC分类号： C12Q122
CPC分类号： A61L2/28
摘要： A process challenge device and method of using the same is presented for testing the efficiency of various sterilization procedures on objects to be sterilized. The device is assembled from two tapered end portions having opposite open ends and an open pathway extending therethrough. A central chamber in the open pathway receives a Biological Indicator for testing the efficiency of the sterilization process. The device is ideal for testing a sterilization process on elongated objects such as tubing, at any point along their length. The coupling structures on the end portions provide the device with single-use security.
摘要翻译： 提出了一种过程挑战装置及其使用方法，用于测试对待消毒物品的各种灭菌程序的效率。 该装置由具有相对开口端的两个锥形端部和延伸通过其的开放通道组装。 开放通道中的中央室接收用于测试灭菌过程效率的生物指示器。 该设备非常适用于在细长物体（如管道）上沿着其长度的任何点测试灭菌过程。 端部上的耦合结构为设备提供一次性安全性。
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