-
1.发明申请公开(公告)号：US20050137387A1
公开(公告)日：2005-06-23
申请号：US10780507
申请日：2004-02-17
申请人： James Mullins , Allen Rodrigo , Gerald Learn , Fusheng Li , David Nickle , Mark Jensen
发明人： James Mullins , Allen Rodrigo , Gerald Learn , Fusheng Li , David Nickle , Mark Jensen
CPC分类号： C07K14/005 , C07H21/04 , C12N7/00 , C12N2740/16021 , C12N2740/16122 , C12N2740/16222 , C12N2770/24222 , C12Q1/703
摘要： The present invention is directed to ancestral and COT nucleic acid and amino acid sequences, methods for producing such sequences and uses thereof, including prophylactic and diagnostic uses.
摘要翻译： 本发明涉及祖代和COT核酸和氨基酸序列，用于产生此类序列及其用途的方法，包括预防和诊断用途。
-
2.发明申请Conserved-Element Vaccines and Methods for Designing Conserved-Element Vaccines 审中-公开保守元素疫苗和设计保护性元素疫苗的方法公开(公告)号：US20110269937A1
公开(公告)日：2011-11-03
申请号：US13097592
申请日：2011-04-29
申请人： James Mullins , David Nickle , Morgane Rolland
发明人： James Mullins , David Nickle , Morgane Rolland
摘要： Embodiments of the present invention include conserved-element vaccines and methods for designing and producing conserved-element vaccines. A conserved-element vaccine (“CEVac”) is a recombinant and/or synthetic vaccine that incorporates only highly conserved epitopes from an observed set of pathogen variants. The conserved epitopes are identified computationally by aligning biopolymer sequences, such as concatenated polypeptide sequences that together represent a pathogen proteome, corresponding to an observed set of pathogen variants, and computationally selecting conserved subsequences according to a number of subsequence-selection criteria. These subsequence-selection criteria may include a minimum conserved-subsequence length, a threshold frequency of occurrence of a particular monomer at each conserved, single-monomer position within a conserved subsequence, a threshold combined occurrence for a set of allowable variant monomers at a particular conserved, variable position within a conserved subsequence, and a maximum number of variable positions within a subsequence. A set of conserved subsequences identified according to the subsequence-selection criteria are then filtered to remove subsequences identical to, or too similar to, naturally-occurring host subsequences, and are then assembled into expression vectors for incorporation into microbial hosts for biosynthesis of a recombinant CEVac or assembled into one or more synthetic constructs for a synthetic CEVac.
摘要翻译： 本发明的实施方案包括保守元件疫苗和用于设计和生产保守元件疫苗的方法。 保守元件疫苗（“CEVac”）是重组和/或合成疫苗，其仅包含观察到的一组病原体变体的高度保守的表位。 通过对齐生物聚合物序列，例如一起代表病原体蛋白质组的连接多肽序列，对应于观察到的一组病原体变体，并且根据多个子序列选择标准计算选择保守的亚序列，可以计算地鉴定保守的表位。 这些子序列选择标准可以包括最小保守序列长度，在保守子序列内的每个保守的单个单体位置处特定单体出现的阈值频率，特定的一组允许的变体单体的阈值组合发生 保守的子序列内的保守的，可变的位置，以及子序列内可变位置的最大数目。 然后将根据子序列选择标准鉴定的一组保守的亚序列过滤以去除与天然存在的宿主亚序列相同或类似于的天然存在的宿主亚序列的亚序列，然后将其组装到表达载体中，以结合到微生物宿主中用于生物合成重组 CEVac或组装成合成CEVac的一种或多种合成构建体。
-
3.发明申请Conserved-element vaccines and methods for designing conserved-element vaccines 审中-公开保守元素疫苗和设计保护性元素疫苗的方法公开(公告)号：US20090092628A1
公开(公告)日：2009-04-09
申请号：US11713474
申请日：2007-03-02
申请人： James Mullins , David Nickle , Morgane Rolland
发明人： James Mullins , David Nickle , Morgane Rolland
摘要： Embodiments of the present invention include conserved-element vaccines and methods for designing and producing conserved-element vaccines. A conserved-element vaccine (“CEVac”) is a recombinant and/or synthetic vaccine that incorporates only highly conserved epitopes from an observed set of pathogen variants. The conserved epitopes are identified computationally by aligning biopolymer sequences, such as concatenated polypeptide sequences that together represent a pathogen proteome, corresponding to an observed set of pathogen variants, and computationally selecting conserved subsequences according to a number of subsequence-selection criteria. These subsequence-selection criteria may include a minimum conserved-subsequence length, a threshold frequency of occurrence of a particular monomer at each conserved, single-monomer position within a conserved subsequence, a threshold combined occurrence for a set of allowable variant monomers at a particular conserved, variable position within a conserved subsequence, and a maximum number of variable positions within a subsequence. A set of conserved subsequences identified according to the subsequence-selection criteria are then filtered to remove subsequences identical to, or too similar to, naturally-occurring host subsequences, and are then assembled into expression vectors for incorporation into microbial hosts for biosynthesis of a recombinant CEVac or assembled into one or more synthetic constructs for a synthetic CEVac.
摘要翻译： 本发明的实施方案包括保守元件疫苗和用于设计和生产保守元件疫苗的方法。 保守元件疫苗（“CEVac”）是重组和/或合成疫苗，其仅包含观察到的一组病原体变体的高度保守的表位。 通过对齐生物聚合物序列，例如一起代表病原体蛋白质组的连接多肽序列，对应于观察到的一组病原体变体，并且根据多个子序列选择标准计算选择保守的亚序列，可以计算地鉴定保守表位。 这些子序列选择标准可以包括最小保守序列长度，在保守子序列内的每个保守的单个单体位置处特定单体出现的阈值频率，特定的一组允许的变体单体的阈值组合发生 保守的子序列内的保守的，可变的位置，以及子序列内可变位置的最大数目。 然后将根据子序列选择标准鉴定的一组保守的亚序列过滤以除去与天然存在的宿主亚序列相同或类似于的天然存在的宿主亚序列的亚序列，然后将其组装到表达载体中，以结合到微生物宿主中用于生物合成重组 CEVac或组装成合成CEVac的一种或多种合成构建体。
-
-
搜索结果
3 条记录 (耗时 0.026 秒)
