公开(公告)号：US20160209395A1

公开(公告)日：2016-07-21

申请号：US14915560

申请日：2014-08-29

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka ,  Kei Kobayashi

IPC: G01N33/487 ,  G01N27/333 ,  G01N27/327

CPC classification number: G01N33/48728 ,  G01N27/327 ,  G01N27/3335 ,  G01N2500/10

Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).

Abstract translation: 1.一种平面膜片钳装置，其特征在于，包括：电绝缘基板（2），具有具有单元排列区域的第一表面和所述相对表面的第二表面，并且在所述单元排列区域中不具有通孔的通孔（3） 但通过液体; 设置在所述电绝缘基板（2）的第一表面侧（2S）处以能够与所述通孔（3）连通并保持第一导电液体的第一储存器（6） 布置成能够通过第一导电液体与第一储存器（6）导电的第一电极部分（7）; 设置在电绝缘基板的第二表面侧（2S'）处以能够与通孔（3）连通并保持第二导电液体的第二储存器（6'）; 布置成能够通过第二导电液体与第二储存器（6'）导电的第二电极部分（7'）; 连接到第二储存器（6'）并将第二导电液体供应到第二储存器的供应路径（8） 连接到第二储存器并从第二储存器（6'）排放第二导电液体的排出路径（9）; 以及设置在所述供给路径和/或排出路径中的能够允许或停止所述第二导电液体的流动的阀门（10），并且还能够允许或停止所述第二储存器（6'）和所述第二储存器 电极部分（7'）。

公开(公告)号：US09977009B2

公开(公告)日：2018-05-22

申请号：US14915560

申请日：2014-08-29

Applicant: Japan Science and Technology Agency

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka ,  Kei Kobayashi

IPC: B01L1/00 ,  B01L3/00 ,  C12M1/00 ,  G01N33/487 ,  G01N27/327 ,  G01N27/333

CPC classification number: G01N33/48728 ,  G01N27/327 ,  G01N27/3335 ,  G01N2500/10

Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid;a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).