公开(公告)号：US20240219370A1

公开(公告)日：2024-07-04

申请号：US18365172

申请日：2023-08-03

Applicant: President and Fellows of Harvard College

Inventor： Donhee Ham ,  Jeffrey T. Abbott ,  Hongkun Park ,  Wenxuan Wu

IPC: G01N33/487 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  B82Y15/00 ,  C12M1/42 ,  C12N13/00 ,  G01N27/00

CPC classification number: G01N33/48728 ,  A61B5/053 ,  A61B5/24 ,  A61N1/18 ,  C12M35/02 ,  C12N13/00 ,  G01N27/00 ,  A61B2562/0285 ,  B82Y15/00

Abstract: Methods and systems for stimulating and monitoring electrogenic cells are described. Some systems for stimulating electrogenic cells are based on the injection of electric currents into the cells via electrodes connected to the cells. Such stimulators may comprise an impedance element having an input terminal and an output terminal coupled to an electrode, and a voltage follower coupled between the input terminal and the output terminal of the impedance element, the voltage follower being configured to maintain a substantially constant voltage between the input terminal and the output terminal of the impedance element. The impedance element may comprise one or more switched capacitors at least in some embodiments. In some embodiments, the voltage follower may be implemented using a source follower.

公开(公告)号：US11994484B2

公开(公告)日：2024-05-28

申请号：US17703783

申请日：2022-03-24

Applicant: The Regents of the University of California

Inventor： Kevin Freedman ,  Vinay Sharma

IPC: G01N27/06 ,  G01N27/447 ,  G01N33/487 ,  G01N11/04

CPC classification number: G01N27/06 ,  G01N33/48728 ,  G01N11/04 ,  G01N27/447

Abstract: A method includes providing a fluid to a structure including an aperture, applying a voltage signal to a circuit that includes the fluid, applying a substantially periodic pressure signal to the fluid, detecting a current signal in the circuit as an analyte passes through the aperture in response to the substantially periodic pressure signal, and processing the current signal and the substantially periodic pressure signal to determine a switch time and a release time for the analyte. An apparatus includes a structure including an aperture to receive a fluid, a voltage source to provide a voltage signal to an electronic circuit having a path that includes the aperture, a pressure signal generator to provide a substantially periodic pressure signal to the fluid, and a system to process the periodic pressure signal and a current signal induced in the electronic circuit.

公开(公告)号：US20240168041A1

公开(公告)日：2024-05-23

申请号：US18282989

申请日：2022-04-14

Applicant: Georgia Tech Research Corporation ,  Emory University

Inventor： Riley E. Perszyk ,  Craig Richard Forest ,  Andrew Jenkins ,  Stephen Traynelis ,  Mighten C. Yip

IPC: G01N35/00 ,  G01N33/487

CPC classification number: G01N35/00584 ,  G01N33/48728

Abstract: An exemplary embodiment of the present disclosure provides a system comprising a pipette tip manipulable between a sample area and an active agent exposure zone and a controller configured to manipulate the pipette tip between the sample area and the active agent exposure zone, wherein when the pipette tip comprises at least a portion of a biological sample target, the controller is further configured to isolate at least a portion of the biological sample target from one or more biological sample targets. Also disclosed is a method for detecting an effect of an active agent on a cell, by forming a high-resistance seal between a cell and an apertured surface, isolating at least a portion of the cell, translocating the apertured surface in one or more translations within an x-plane, a y-plane, a z-plane, or combinations thereof, and exposing the portion of the cell to an active agent.

公开(公告)号：US11828751B2

公开(公告)日：2023-11-28

申请号：US17001243

申请日：2020-08-24

Applicant: Panasonic Intellectual Property Management Co., Ltd.

Inventor： Kiyotaka Tsuji ,  Yumiko Kato

IPC: C12Q1/00 ,  G01N33/50 ,  G01N33/487

CPC classification number: G01N33/5061 ,  G01N33/48728 ,  G01N2800/709

Abstract: A method for evaluating drug responsiveness includes disposing a myocardial cell produced through differential induction onto a board including an electrode, administering a drug to the myocardial cell, continuously applying, after the drug is administered, a pulse current or a pulse voltage to the myocardial cell through the electrode for a certain period of time, measuring, after the certain period of time has elapsed, a pulsation characteristic of the myocardial cell, and evaluating responsiveness of the myocardial cell to the drug on a basis of the pulsation characteristic.

公开(公告)号：US11707748B2

公开(公告)日：2023-07-25

申请号：US17121285

申请日：2020-12-14

Applicant: Regents of the University of Minnesota

Inventor： Sang-Hyun Oh ,  Steven John Koester

IPC: B03C5/00 ,  G01N27/447 ,  G01N33/487 ,  B03C5/02

CPC classification number: B03C5/005 ,  B03C5/026 ,  G01N27/44721 ,  G01N27/44756 ,  G01N33/48721 ,  G01N33/48728 ,  B03C2201/26

Abstract: Devices, systems, and methods for applying a dielectrophoretic force on a particle include: a cell defining at least one channel for confining the particle; and a first electrode and a second electrode electrically isolated from the first electrode, at least one of the first and second electrodes being formed from a two-dimensional (2D) material providing an atomically sharp edge. The first and second electrodes are arranged sufficiently close to one another and sufficiently close to the channel such that application of a sufficient voltage across the first and second electrodes generates an electric field in at least part of the channel, the electric field having an electric field gradient sufficient to apply the dielectrophoretic force on the particle in the channel.

公开(公告)号：US20230204563A1

公开(公告)日：2023-06-29

申请号：US17584397

申请日：2022-01-26

Applicant: National Tsing Hua University

Inventor： Yu-Lin Wang ,  Shu-Yi Tsai

IPC: G01N33/487 ,  G01N33/543

CPC classification number: G01N33/48728 ,  G01N33/5438 ,  G01N27/4145

Abstract: A sensing device is provided. The sensing device includes a transistor, a disposable electrode, and a remote electrode. The transistor includes an extended gate, source and drain. The remote electrode is configured to receive a reference voltage. The disposable electrode is coupled between the transistor and the remote electrode. The disposable electrode includes a proximal end and a distal end. The proximal end of the disposable electrode is coupled to the extended gate of the transistor. The distal end of the disposable electrode is coupled to the remote electrode. The disposable electrode is adapted to load a cell and receive a membrane potential of the cell. The disposable electrode provides a gate voltage to the extended gate based on the change of the membrane potential and the reference voltage. The transistor provides different transistor currents at the drain based on the change of the gate voltage.

公开(公告)号：US20190211370A1

公开(公告)日：2019-07-11

申请号：US16241680

申请日：2019-01-07

Applicant: IMPETUX OPTICS, S.L.

Inventor： Arnau FARRÉ FLAQUER ,  Ferran MARSÀ SAMPER

IPC: C12Q1/02 ,  G01N21/71 ,  G01N25/48

CPC classification number: C12Q1/02 ,  G01N21/718 ,  G01N25/482 ,  G01N33/48728 ,  G01N2201/06106 ,  G02B21/32

Abstract: A method to label as defective a measure of an optical-trap force, that is exerted on a trapped particle located inside a living, dispersive, viscoelastic medium, including operations of: (i) determining a calibration constant between the optical trap forces and the sensed voltages; (ii) determining a first calibration function of the frequency of the particle oscillation with the active-passive procedure; (iii) computing a second calibration function of the frequency as the quotient between the calibration constant and the first calibration function; (iv) computing an energy function of the frequency as the product of the thermal energy of the trapped particle and the second calibration function; (v) checking whether the energy function converges to the thermal energy of the trapped particle as the frequency increases; (vi) if there is no such convergence, then label as defective the measure of the optical-trap force.

公开(公告)号：US20180348114A1

公开(公告)日：2018-12-06

申请号：US16101336

申请日：2018-08-10

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Hsuan-Yu Huang ,  Chih-Pin Chang ,  Kuang-Li Huang ,  Yu-Chiao Chi

IPC: G01N15/14 ,  G02B21/34 ,  G06T7/00 ,  G02B21/24 ,  G01N33/487

CPC classification number: G01N15/1475 ,  A61B10/0058 ,  G01N15/1434 ,  G01N33/48728 ,  G01N2015/1006 ,  G01N2015/144 ,  G01N2015/1486 ,  G01N2015/1497 ,  G02B21/244 ,  G02B21/34 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30241

Abstract: Embodiments disclose a device for testing biological specimen. The device includes a sample carrier and a detachable cover. The sample carrier includes a specimen holding area. The detachable cover is placed on top of the specimen holding area. The detachable cover includes a magnifying component configured to align with the specimen holding area. The focal length of the magnifying component is from 0.1 mm to 8.5 mm. The magnifying component has a linear magnification ratio of at least 1. Some embodiments further include a multi-camera configuration. These embodiments include a first camera module and a second camera module arranged to capture one or more images of the first holding area and the second holding area, respectively. The processor may perform different analytic processes on the captured images of different holding areas to determine an outcome with regard to the biological specimen.

公开(公告)号：US20180209987A1

公开(公告)日：2018-07-26

申请号：US15745044

申请日：2016-07-28

Applicant: The Regents of the University of California

Inventor： Evan W. Miller ,  Yi-Lin Huang ,  Alison S. Walker

IPC: G01N33/58 ,  G01N33/487 ,  C07F7/08 ,  C09B69/00 ,  C09B69/06 ,  G01N15/14

CPC classification number: G01N33/582 ,  C07F7/0816 ,  C09B69/008 ,  C09B69/06 ,  G01N15/1475 ,  G01N33/48728 ,  G01N2015/1006

Abstract: The disclosure provides for provides for water-soluble near-infra red (NIR) emissive compounds, processes of making the compounds thereof, and use of the compounds therein.

公开(公告)号：US20180194914A1

公开(公告)日：2018-07-12

申请号：US15742027

申请日：2016-06-12

Applicant: Nanosniff Technologies Pvt. Ltd.

Inventor： Nitin Kale ,  Sumona Dhara ,  Dadasaheb Sangave ,  Kapil Bardeja

IPC: C08J7/12 ,  C07K16/18 ,  A61K39/395

CPC classification number: C08J7/12 ,  A61K39/39533 ,  C07K16/18 ,  C08G77/388 ,  C08L23/06 ,  C08L25/06 ,  C08L27/06 ,  C08L33/12 ,  G01N33/48728 ,  G01N33/48785 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393

Abstract: An asymmetric immobilization process that immobilizes a one or more receptor biomolecules on one or more solid surfaces is provided. The asymmetric immobilization process includes the following steps: (i) activating the one or more solid surfaces using an oxidizing agent; (ii) treating the one or more solid surfaces with aminosilane to obtain a one or more amine functionalized solid surfaces; (iii) treating the one or more receptor biomolecules with a 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) to obtain a one or more site-specific EDC activated receptor biomolecules; and (iv) immobilizing the one or more site-specific EDC activated receptor biomolecules on a first layer of the one or more amine functionalized solid surfaces, wherein the immobilizing comprises treating the one or more site-specific EDC activated receptor biomolecules with amine groups of the first layer of the one or more amine functionalized solid surfaces to form a covalent amide bond.