公开(公告)号：US10114005B2

公开(公告)日：2018-10-30

申请号：US14366708

申请日：2012-12-05

Applicant: Japan Science and Technology Agency

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Senthil Kumar Obuliraj ,  Yasutaka Nagaoka

IPC: G01N33/487 ,  G01N27/416

Abstract: An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current.Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.

公开(公告)号：US09829477B2

公开(公告)日：2017-11-28

申请号：US14429035

申请日：2013-03-21

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Miho Saitoh ,  Yasutaka Nagaoka

IPC: C12M1/32 ,  G01N33/487 ,  C12M1/00 ,  C12M1/26

CPC classification number: G01N33/48728 ,  C12M23/04 ,  C12M23/12 ,  C12M23/38 ,  C12M25/06 ,  C12M33/00 ,  C12M33/04 ,  C12M35/02 ,  C12N5/0619

Abstract: A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network. The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.

公开(公告)号：US10208284B2

公开(公告)日：2019-02-19

申请号：US15113153

申请日：2015-01-23

Applicant: Japan Science and Technology Agency

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka

IPC: C12M1/00 ,  C12N5/079 ,  C12M1/12 ,  C12N13/00 ,  C12M1/32 ,  C12M3/06

Abstract: Provided is a device for seeding cells in a plurality of cell arrangement areas in a simple manner and a short period of time. A seeding and culturing device (1) for cells capable of forming a nerve network, the device comprising a cell-culturing substrate (2) having a plurality of cell arrangement areas (8) enclosed by a plurality of projecting parts, and a flow channel substrate (3) arranged on the cell-cultivating substrate (2) and having a plurality of through-holes (14), wherein the through-holes (14) are configured so as to provide flow channels in which the upper surface side of the substrate is an entrance (15) and the lower surface side of the substrate is an exit, and the exit (16) of the flow channels is positioned above any of the cell arrangement areas.

公开(公告)号：US09977009B2

公开(公告)日：2018-05-22

申请号：US14915560

申请日：2014-08-29

Applicant: Japan Science and Technology Agency

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka ,  Kei Kobayashi

IPC: B01L1/00 ,  B01L3/00 ,  C12M1/00 ,  G01N33/487 ,  G01N27/327 ,  G01N27/333

CPC classification number: G01N33/48728 ,  G01N27/327 ,  G01N27/3335 ,  G01N2500/10

Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid;a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).

公开(公告)号：US20150233890A1

公开(公告)日：2015-08-20

申请号：US14429035

申请日：2013-03-21

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Miho Saitoh ,  Yasutaka Nagaoka

IPC: G01N33/487 ,  C12M1/00 ,  C12M1/26 ,  C12M1/32

CPC classification number: G01N33/48728 ,  C12M23/04 ,  C12M23/12 ,  C12M23/38 ,  C12M25/06 ,  C12M33/00 ,  C12M33/04 ,  C12M35/02 ,  C12N5/0619

Abstract: Provided are a culture device for neuronal network formation that makes it possible to construct a neuronal network while restricting movement of neurons grown in the culture medium, and a means for the use thereof. A culture device for neuronal network formation in which is formed a cell fixation part surrounded by a plurality of projections on a flat substrate that can be filled with cell culture medium, and in which (1) there are established spaces having widths such that the neuron cells cannot pass through between the plurality of projections, (2) the inner diameter is such that the cell fixation part can house the cells of from one to a number of neurons, (3) the substrate surface, which is the bottom of the cell fixation part, is coated by an extracellular matrix-forming substance and/or provided with minute through-holes for suctioning culture medium by a suction device on the lower side of the substrate surface.

Abstract translation: 提供了一种用于神经元网络形成的培养装置，其可以构建神经元网络，同时限制在培养基中生长的神经元的运动，以及用于其的手段。 一种用于神经元网络形成的培养装置，其形成有可以填充有细胞培养基的平坦基底上的多个突起包围的细胞固定部分，其中（1）具有宽度使得神经元 细胞不能通过多个突起之间，（2）内径使得细胞固定部分能够容纳从一个到多个神经元的细胞，（3）作为细胞底部的底物表面 固定部分由细胞外基质形成物质涂覆和/或设置有用于通过基底表面下侧的抽吸装置吸引培养基的微小通孔。

公开(公告)号：US20170002315A1

公开(公告)日：2017-01-05

申请号：US15113153

申请日：2015-01-23

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka

IPC: C12N5/079 ,  C12M1/12 ,  C12M3/06

CPC classification number: C12N5/0618 ,  C12M23/04 ,  C12M23/16 ,  C12M25/00 ,  C12M25/06 ,  C12M33/06

Abstract: Provided is a device for seeding cells in a plurality of cell arrangement areas in a simple manner and a short period of time. A seeding and culturing device (1) for cells capable of forming a nerve network, the device comprising a cell-culturing substrate (2) having a plurality of cell arrangement areas (8) enclosed by a plurality of projecting parts, and a flow channel substrate (3) arranged on the cell-cultivating substrate (2) and having a plurality of through-holes (14), wherein the through-holes (14) are configured so as to provide flow channels in which the upper surface side of the substrate is an entrance (15) and the lower surface side of the substrate is an exit, and the exit (16) of the flow channels is positioned above any of the cell arrangement areas.

Abstract translation: 提供了一种用于以简单的方式和短时间段在多个单元布置区域中种植单元的装置。 一种用于形成神经网络的细胞的接种和培养装置（1），所述装置包括具有由多个突出部分包围的多个细胞排列区域（8）的细胞培养基板（2），以及流路 配置在细胞培养基板（2）上并具有多个通孔（14）的基板（3），其中，所述通孔（14）构成为提供流路，其中， 衬底是入口（15），衬底的下表面侧是出口，并且流动通道的出口（16）位于任何单元布置区域上方。

公开(公告)号：US20160209395A1

公开(公告)日：2016-07-21

申请号：US14915560

申请日：2014-08-29

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Yasutaka Nagaoka ,  Kei Kobayashi

IPC: G01N33/487 ,  G01N27/333 ,  G01N27/327

CPC classification number: G01N33/48728 ,  G01N27/327 ,  G01N27/3335 ,  G01N2500/10

Abstract: A planar patch clamp device comprising: an electrical insulating substrate (2) having a first surface having a cell arrangement region and a second surface of the opposite surface and having a through hole (3) in the cell arrangement region which does not pass cells, but pass liquid; a first reservoir (6) provided at the first surface side (2S) of the electrical insulating substrate (2) to be able to communicate with the through hole (3) and hold a first conductive liquid; a first electrode part (7) arranged to be able to be electrically conductive with the first reservoir (6) through the first conductive liquid; a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).

Abstract translation: 1.一种平面膜片钳装置，其特征在于，包括：电绝缘基板（2），具有具有单元排列区域的第一表面和所述相对表面的第二表面，并且在所述单元排列区域中不具有通孔的通孔（3） 但通过液体; 设置在所述电绝缘基板（2）的第一表面侧（2S）处以能够与所述通孔（3）连通并保持第一导电液体的第一储存器（6） 布置成能够通过第一导电液体与第一储存器（6）导电的第一电极部分（7）; 设置在电绝缘基板的第二表面侧（2S'）处以能够与通孔（3）连通并保持第二导电液体的第二储存器（6'）; 布置成能够通过第二导电液体与第二储存器（6'）导电的第二电极部分（7'）; 连接到第二储存器（6'）并将第二导电液体供应到第二储存器的供应路径（8） 连接到第二储存器并从第二储存器（6'）排放第二导电液体的排出路径（9）; 以及设置在所述供给路径和/或排出路径中的能够允许或停止所述第二导电液体的流动的阀门（10），并且还能够允许或停止所述第二储存器（6'）和所述第二储存器 电极部分（7'）。

公开(公告)号：US20140339102A1

公开(公告)日：2014-11-20

申请号：US14366708

申请日：2012-12-05

Applicant: Japan Science and Technology Agency

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Senthil Kumar Obuliraj ,  Yasutaka Nagaoka

IPC: G01N33/487 ,  G01N27/416

CPC classification number: G01N33/48728 ,  G01N27/416

Abstract: An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current.Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.

Abstract translation: 本发明的目的在于抑制平板式钳夹装置中的膜电位的变化，以降低噪声电流，从而能够精确地测量离子通道电流。 公开了一种平面膜片钳装置，包括：具有一个或多个细通孔的电绝缘衬底; 保持设置在通孔的两个表面侧的导电液体的液体储存器; 和设置在液体储存器中的可激励电极部分; 这些电极部分包括：（a）电极容器，其至少一部分由无机多孔材料制成，（b）在贵金属Nm表面上形成氯化物NmCl层的电极，（c ）以饱和浓度溶解含有NmCl和碱金属氯化物的电极溶液。