公开(公告)号：US20130164797A1

公开(公告)日：2013-06-27

申请号：US13721481

申请日：2012-12-20

申请人： Jasmin GIELEN ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

发明人： Jasmin GIELEN ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

IPC分类号： C12P7/64

CPC分类号： C12P7/6436 ,  C12N9/001 ,  C12P13/04 ,  C12Y103/99003

摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyses one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyses one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

摘要翻译： 提供了一种从水性培养基中改进疏水性有机溶液分离的方法。 该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。 代谢活性细胞的活性降低与野生型活性细胞相比，活性降低至少是一种催化脂肪酸β-氧化反应的一种酶。 本发明还提供了一种代谢活性细胞，其与野生型相比具有降低的活性，所述活性细胞能催化脂肪酸的β-氧化反应之一，包括选自FadA，FadB，FadD， FadL和FadE及其变体。

公开(公告)号：US09249435B2

公开(公告)日：2016-02-02

申请号：US13721481

申请日：2012-12-20

申请人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

发明人： Jasmin Gielen ,  Thomas Haas ,  Hans-Georg Hennemann ,  Steffen Schaffer ,  Mirja Wessel ,  Katharina Berse ,  Marion Schiebelhut ,  Sabine Hafkemeyer ,  Markus Poetter ,  Frank Erhardt

IPC分类号： C12P13/04 ,  C12P7/64 ,  C12N9/02

CPC分类号： C12P7/6436 ,  C12N9/001 ,  C12P13/04 ,  C12Y103/99003

摘要： A process for the improved separation of a hydrophobic organic solution from an aqueous culture medium is provided. The process includes preparing an aqueous culture medium of a metabolically active cell having a decreased activity; contacting of the aqueous culture medium with a hydrophobic organic solution comprising a substrate for biotransformation; conducting a biotransformation of the substrate; and separating the hydrophobic organic solution comprising a biotransformed substrate from the aqueous culture medium. The decreased activity of the metabolically active cell is in comparison to a wild-type of the active cell and the decreased activity is of at least of one enzyme that catalyzes one reaction of β-oxidation of fatty acids. The invention further provides a metabolically active cell that has a decreased activity, compared to its wild-type, of an enzyme that catalyzes one of the reactions of the β-oxidation of fatty acids, including an enzyme selected from FadA, FadB, FadD, FadL and FadE as well as variants thereof.

摘要翻译： 提供了一种从水性培养基中改善疏水性有机溶液分离的方法。 该方法包括制备活性降低的代谢活性细胞的水性培养基; 使含水培养基与包含用于生物转化的底物的疏水性有机溶液接触; 进行基底的生物转化; 并从含水培养基中分离包含生物转化底物的疏水性有机溶液。 与野生型活性细胞相比，代谢活性细胞的活性降低，活性降低至少是催化脂肪酸一次反应的一种酶。 本发明进一步提供了一种代谢活性细胞，其与野生型相比具有降低的活性，其酶催化脂肪酸的一种反应的反应，包括选自FadA，FadB，FadD的酶 ，FadL和FadE及其变体。