公开(公告)号：US07691389B2

公开(公告)日：2010-04-06

申请号：US11366851

申请日：2006-03-01

申请人： Jay Gregory Calvert ,  Michael G Sheppard ,  Siao-Kun W. Welch

发明人： Jay Gregory Calvert ,  Michael G Sheppard ,  Siao-Kun W. Welch

IPC分类号： A61K39/12

CPC分类号： C07K14/005 ,  A61K2039/525 ,  A61K2039/53 ,  C12N15/86 ,  C12N2770/10022 ,  C12N2770/10043

摘要： The invention provides isolated polynucleotide molecules, including plasmids; viral vectors; and transfected host cells that comprise a DNA sequence encoding an infectious RNA sequence encoding a North American PRRS virus; and also North American PRRS viruses encoded thereby. The invention further provides isolated infectious RNA molecules encoding a North American PRRS virus. The invention also provides isolated polynucleotide molecules, infectious RNA molecules, viral vectors, and transfected host cells encoding genetically-modified North American PRRS viruses; and genetically-modified North American PRRS viruses encoded thereby. The invention also provides vaccines comprising such plasmids, RNA molecules, viral vectors, and North American PRRS viruses, and methods of using these vaccines in swine and in other animals. Also provided are isolated polynucleotide molecules, viral vectors, and transfected host cells that comprise a nucleotide sequence encoding a peptide of a North American PRRS virus. These viral vectors and transfected host cell lines are useful in providing peptides to compensate for mutated peptide coding sequences of DNA sequences encoding genetically-modified North American PRRS viruses so that functional virions can be generated.

摘要翻译： 本发明提供分离的多核苷酸分子，包括质粒; 病毒载体; 和包含编码编码北美PRRS病毒的感染性RNA序列的DNA序列的转染宿主细胞; 以及由此编码的北美PRRS病毒。 本发明还提供编码北美PRRS病毒的分离的感染性RNA分子。 本发明还提供分离的多核苷酸分子，感染性RNA分子，病毒载体和编码经遗传修饰的北美PRRS病毒的转染宿主细胞; 和由此编码的转基因北美PRRS病毒。 本发明还提供包含这样的质粒，RNA分子，病毒载体和北美PRRS病毒的疫苗，以及在猪和其他动物中使用这些疫苗的方法。 还提供了分离的多核苷酸分子，病毒载体和包含编码北美PRRS病毒的肽的核苷酸序列的转染宿主细胞。 这些病毒载体和转染的宿主细胞系可用于提供肽以补偿编码经遗传修饰的北美PRRS病毒的DNA序列的突变的肽编码序列，从而可以产生功能性病毒体。