公开(公告)号：US20110306055A1

公开(公告)日：2011-12-15

申请号：US12935201

申请日：2010-02-24

申请人： Jean-Francois Haince ,  Guillaume Beaudry ,  Genevieve Garon ,  Michel Houde ,  Timothy J. Holzer ,  Martin Beaulieu ,  Nicolas Bertrand

发明人： Jean-Francois Haince ,  Guillaume Beaudry ,  Genevieve Garon ,  Michel Houde ,  Timothy J. Holzer ,  Martin Beaulieu ,  Nicolas Bertrand

IPC分类号： C12Q1/68

CPC分类号： G01N33/57419 ,  C12Q1/34 ,  C12Q1/527 ,  G01N33/573 ,  G01N2800/52

摘要： The present invention provides a novel method for diagnosing, monitoring, prognosing and staging Lymph Node (LN) status in colorectal cancer (CRC) that is more sensitive and accurate than conventional detection technologies such as histopathology. The Guanylyl Cyclase C (GCC) gene is specifically expressed in apical epithelial cells of the GI tract from the duodenum to the rectum and the detection of GCC mRNA in LNs is indicative of the presence of metastases. Quantitative RT-PCR (RT-qPCR) detection of GCC mRNA to identify the presence of colorectal cancer (CRC) cells in LNs has the potential to aid in CRC staging. When used in combination with glucuronidase B (GUSB), accurate quantification of GCC can be achieved with less than a 2-fold variation between intact and highly degraded RNA specimens. The invention also relates to a newly designed GCC/GUSB assay that uses relative quantification having improved prognostic value for time to recurrence and relapse-free survival in Stage I or II colon cancer patients. The GCC/GUSB assay also improves the statistical power of prognosis stratification for relative risk of recurrence and relapse-free survival.

摘要翻译： 本发明提供了一种用于诊断，监测，预后和分期结直肠癌（CRC）中的淋巴结（LN）状态的新方法，其比常规检测技术如组织病理学更灵敏和准确。 Guanylyl Cyclase C（GCC）基因在十二指肠至直肠胃肠道的顶端上皮细胞中特异性表达，LN检测GCC mRNA表示存在转移灶。 定量RT-PCR（RT-qPCR）检测GCC mRNA以鉴定LN中结肠直肠癌（CRC）细胞的存在有助于CRC分期。 当与葡糖醛酸糖苷酶B（GUSB）组合使用时，可以在完整和高度降解的RNA标本之间以小于2倍的变化实现GCC的准确定量。 本发明还涉及一种新设计的GCC / GUSB测定法，其使用相对定量，其具有改善I期或II期结肠癌患者的复发和无复发存活时间的预后价值。 GCC / GUSB测定还提高了复发和无复发生存相对风险的预后分层的统计学功能。

公开(公告)号：US06561198B1

公开(公告)日：2003-05-13

申请号：US09623456

申请日：2000-11-06

申请人： Bernard Drevillon ,  Nicolas Bertrand ,  Jean-Christophe Rostaing

发明人： Bernard Drevillon ,  Nicolas Bertrand ,  Jean-Christophe Rostaing

IPC分类号： C25F500

CPC分类号： C23G5/00 ,  B08B7/0035

摘要： In this method for the surface treatment of a metal part (12) for the purpose of deoxidizing it and/or cleaning it, a sealed chamber (16), in which the part to be treated is placed, is filled with a low-pressure reducing gas mixture, a static magnetic field is created in a region of the chamber (16) separate from the region in which the part (12) to be treated is placed and the gas mixture is excited by means of an electromagnetic wave injected into the chamber (16) so as to generate a treatment plasma in the gas, the intensity of the static magnetic field corresponding to electron cyclotron resonance established in the chamber in a distributed manner.

摘要翻译： 在用于对其进行脱氧和/或清洁的金属部件（12）的表面处理的方法中，将待处理部件放置在其中的密封室（16）填充有低压 减少气体混合物，在与被处理部分（12）放置的区域分离的腔室（16）的区域中产生静态磁场，并且气体混合物通过注入到所述待处理的电磁波中而被激发 （16），以在气体中产生处理等离子体，以分布式的方式在腔室中建立对应于电子回旋共振的静磁场的强度。