Method for culturing recombinant cells
    1.
    发明授权
    Method for culturing recombinant cells 失效
    培养重组细胞的方法

    公开(公告)号:US06399331B2

    公开(公告)日:2002-06-04

    申请号:US09728342

    申请日:2000-11-30

    IPC分类号: C12P2102

    CPC分类号: C07K14/59 C07K14/79

    摘要: A method for culturing a recombinant host cell comprising: determining a polypeptide factor for a polypeptide factor-dependent host cell; transforming said host cell with nucleic acid encoding said polypeptide factor; transforming the host cell with nucleic acid encoding a desired protein; and, culturing the transformed host cells in a medium lacking the polypeptide factor.

    摘要翻译: 一种用于培养重组宿主细胞的方法,包括:确定多肽因子依赖性宿主细胞的多肽因子; 用编码所述多肽因子的核酸转化所述宿主细胞; 用编码所需蛋白质的核酸转化宿主细胞; 并且在缺乏多肽因子的培养基中培养转化的宿主细胞。