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公开(公告)号:US06399331B2
公开(公告)日:2002-06-04
申请号:US09728342
申请日:2000-11-30
申请人: Jennie P Mather , Axel Ullrich
发明人: Jennie P Mather , Axel Ullrich
IPC分类号: C12P2102
摘要: A method for culturing a recombinant host cell comprising: determining a polypeptide factor for a polypeptide factor-dependent host cell; transforming said host cell with nucleic acid encoding said polypeptide factor; transforming the host cell with nucleic acid encoding a desired protein; and, culturing the transformed host cells in a medium lacking the polypeptide factor.
摘要翻译: 一种用于培养重组宿主细胞的方法,包括:确定多肽因子依赖性宿主细胞的多肽因子; 用编码所述多肽因子的核酸转化所述宿主细胞; 用编码所需蛋白质的核酸转化宿主细胞; 并且在缺乏多肽因子的培养基中培养转化的宿主细胞。