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145 条记录 (耗时 0.039 秒)

    Methods and cells for expression of recombinant protein products under the transcriptional control of an inducible promoter
    1.
    发明授权
    Methods and cells for expression of recombinant protein products under the transcriptional control of an inducible promoter 有权
    用于在诱导型启动子的转录控制下表达重组蛋白产物的方法和细胞

    公开(公告)号：US06803210B2

    公开(公告)日：2004-10-12

    申请号：US09811933

    申请日：2001-03-19

    申请人： Marc D. Better

    发明人： Marc D. Better

    IPC分类号： C12P2102

    CPC分类号： C12P21/02 ,  C12N15/635 ,  C12N15/70

    摘要： The present invention relates generally to improved methods for the expression of recombinant protein products under the transcriptional control of an inducible promoter, such as an araB promoter, in bacterial host cells that are deficient in one or more of the active transport systems for the inducer of the inducible promoter. The present invention also relates to improved bacterial host cells that are deficient in one or more of the active transport systems for an inducer of an inducible promoter, such as arabinose for an araB promoter, and contain an expression vector encoding a recombinant polypeptide under the transcriptional control of the inducible promoter, such as an araB promoter.

    摘要翻译： 本发明一般涉及用于在细菌宿主细胞中的诱导型启动子如araB启动子的转录控制下表达重组蛋白产物的改进方法，所述细菌宿主细胞缺乏一种或多种用于诱导剂的诱导剂的活性转运体系 诱导型启动子。 本发明还涉及改进的细菌宿主细胞，其缺乏一种或多种用于诱导型启动子的诱导物的活性转运系统，例如araB启动子的阿拉伯糖，并且在转录下含有编码重组多肽的表达载体 诱导型启动子的控制，如araB启动子。

    Methods of producing anti-angiogenic proteins
    2.
    发明授权
    Methods of producing anti-angiogenic proteins 失效
    产生抗血管生成蛋白的方法

    公开(公告)号：US06797488B1

    公开(公告)日：2004-09-28

    申请号：US09589483

    申请日：2000-06-07

    申请人： Vikas P. Sukhatme

    发明人： Vikas P. Sukhatme

    IPC分类号： C12P2102

    CPC分类号： C07K14/78

    摘要： Methods for making proteins with anti-angiogenic properties are disclosed. The system used is a yeast expression system which produces biologically active proteins at high titer.

    摘要翻译： 公开了制备具有抗血管生成特性的蛋白质的方法。 所使用的系统是以高效价产生生物活性蛋白质的酵母表达系统。

    Promoters for expressing genes in a fungal cell
    4.
    发明授权
    Promoters for expressing genes in a fungal cell 失效
    在真菌细胞中表达基因的启动子

    公开(公告)号：US06692940B2

    公开(公告)日：2004-02-17

    申请号：US10281673

    申请日：2002-10-28

    申请人： Randy M. Berka ,  Michael W. Rey ,  Kimberly Brown ,  Stephen H. Brown

    发明人： Randy M. Berka ,  Michael W. Rey ,  Kimberly Brown ,  Stephen H. Brown

    IPC分类号： C12P2102

    CPC分类号： C12N9/2428 ,  C07K14/37 ,  C12N15/80

    摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter foreign to the nucleic acid sequence, wherein the promoter comprises a sequence selected from the group consisting of nucleotides 1 to 3949 of SEQ ID NO. 1, nucleotides 1 to 938 of SEQ ID NO. 2, and nucleotides 1 to 3060 of SEQ ID NO. 3, and a subsequence thereof; and mutant, hybrid, and tandem promoters thereof; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to the isolated promoter sequences and to constructs, vectors, and fungal host cells comprising the promoter sequences operably linked to nucleic acid sequences encoding polypeptides.

    摘要翻译： 本发明涉及生产多肽的方法，其包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含编码多肽的第一核酸序列，其可操作地连接到 第二核酸序列，其包含核酸序列外源的启动子，其中所述启动子包含选自SEQ ID NO：1的核苷酸1至3949的序列。 1，SEQ ID NO：1的核苷酸1至938。 2和SEQ ID NO：1的核苷酸1至3060。 3及其子序列; 及其突变体，杂种和串联启动子; 和（b）从培养基中分离多肽。 本发明还涉及分离的启动子序列和构建体，载体和真菌宿主细胞，其包含可操作地连接到编码多肽的核酸序列的启动子序列。

    Human minor vault protein p193
    5.
    发明授权
    Human minor vault protein p193 有权
    人类小型蛋白质p193

    公开(公告)号：US06555347B1

    公开(公告)日：2003-04-29

    申请号：US09607510

    申请日：2000-06-28

    申请人： Leonard H. Rome ,  Valerie A. Kickhoefer

    发明人： Leonard H. Rome ,  Valerie A. Kickhoefer

    IPC分类号： C12P2102

    CPC分类号： C07K14/47 ,  A61K38/00 ,  C12Q1/6886 ,  C12Q2600/158

    摘要： A polynucleotide molecule encoding human minor vault protein p193, or its complementary strands. A purified and isolated polynucleotide molecule consisting essentially of a nucleotide sequence encoding human minor vault protein p193, or its complementary strands, or a combination of a nucleotide sequence encoding human minor vault protein p193 and its complementary strands.

    摘要翻译： 编码人类小型蛋白质蛋白质p193或其互补链的多核苷酸分子。 纯化和分离的多核苷酸分子，其基本上由编码人次要保管蛋白p193或其互补链的核苷酸序列或编码人次要保管蛋白p193的核苷酸序列及其互补链的组合组成。

    Fused polypeptides
    7.
    发明授权
    Fused polypeptides 有权
    融合多肽

    公开(公告)号：US06531579B1

    公开(公告)日：2003-03-11

    申请号：US09258000

    申请日：1999-02-25

    申请人： Frederic de Sauvage ,  Arnon Rosenthal

    发明人： Frederic de Sauvage ,  Arnon Rosenthal

    IPC分类号： C12P2102

    CPC分类号： C12N9/1205 ,  C07K16/40 ,  C07K2319/00 ,  C12N2799/026

    摘要： The present invention relates to nucleotide sequences, including expressed sequence tags (ESTs), oligonucleotide probes, polypeptides, vectors and host cells expressing, immunoadhesins, agonists and antagonists to human & vertebrate fused.

    摘要翻译： 本发明涉及核苷酸序列，包括表达序列标签（ESTs），寡核苷酸探针，多肽，载体和表达人和脊椎动物融合蛋白的免疫粘附素，激动剂和拮抗剂的宿主细胞。

    GFP-annexin fusion proteins
    8.
    发明授权
    GFP-annexin fusion proteins 失效
    GFP-膜联蛋白融合蛋白

    公开(公告)号：US06511829B1

    公开(公告)日：2003-01-28

    申请号：US08948276

    申请日：1997-10-09

    申请人： Joel D. Ernst

    发明人： Joel D. Ernst

    IPC分类号： C12P2102

    CPC分类号： C07K14/4721 ,  C07K14/43595 ,  C07K2319/00 ,  G01N2333/4718

    摘要： Bifunctional green fluorescent protein (GFP)-annexin fusion proteins combine the inherent strong visible fluorescent properties of GFPs with the anionic phospholipid binding specificity of annexins. Recombinant host cells, especially bacteria, are used to efficiently express the fusion proteins in high yield and soluble form, suitable for rapid, one-step affinity purification. Uses include of selective cellular and biochemical labeling, particularly anionic species, such as selectively labeling apoptotic cells.

    摘要翻译： 双功能绿色荧光蛋白（GFP）-annexin融合蛋白将GFP的固有强可见荧光特性与膜联蛋白的阴离子磷脂结合特异性相结合。 重组宿主细胞，特别是细菌，用于以高产率和可溶性形式有效表达融合蛋白，适用于快速，一步亲和纯化。 使用选择性细胞和生物化学标记，特别是阴离子物质，如选择性标记凋亡细胞。

    Methods for enhancing the production of interferon in cell culture
    9.
    发明授权
    Methods for enhancing the production of interferon in cell culture 失效
    增强细胞培养中干扰素生产的方法

    公开(公告)号：US06489144B1

    公开(公告)日：2002-12-03

    申请号：US09591813

    申请日：2000-06-12

    申请人： Allan S. Lau

    发明人： Allan S. Lau

    IPC分类号： C12P2102

    CPC分类号： C12N9/1205 ,  A61K38/00 ,  C07K14/555 ,  C12N7/00 ,  C12N15/1137 ,  C12N15/67 ,  C12N2310/111 ,  C12N2710/16643 ,  C12N2770/32051 ,  C12P21/02 ,  C12Q1/18

    摘要： Methods for enhancing the production of interferon in animal cell culture are described. These methods rely on the manipulation of the cellular levels of certain inducers of interferon production, in particular cellular levels of double-stranded-RNA-dependent kinase (dsRNA-PKR, or PKR). In cell cultures that overproduce PKR, interferon synthesis is induced to high levels, and significant amounts of interferon can be recovered without conventional induction of interferon by virus.

    摘要翻译： 描述了用于增强动物细胞培养物中干扰素生产的方法。 这些方法依赖于干扰素产生的某些诱导物的细胞水平的操作，特别是双链RNA依赖性激酶（dsRNA-PKR或PKR）的细胞水平。 在过量产生PKR的细胞培养物中，干扰素合成被诱导为高水平，并且可以在不常规诱导病毒感染干扰素的情况下回收大量的干扰素。