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    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris
    1.
    发明授权
    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including protein markers, pathogens and cellular debris 有权
    基于平面光波导的夹层测定传感器和用于检测生物靶标（包括蛋白质标记物，病原体和细胞碎片）的方法

    公开(公告)号：US07541197B2

    公开(公告)日：2009-06-02

    申请号：US11172246

    申请日：2005-06-29

    申请人： Jennifer S. Martinez ,  Basil I. Swanson ,  Karen M. Grace ,  Wynne K. Grace ,  Andrew P. Shreve

    发明人： Jennifer S. Martinez ,  Basil I. Swanson ,  Karen M. Grace ,  Wynne K. Grace ,  Andrew P. Shreve

    IPC分类号： G01N33/551

    CPC分类号： G01N21/648 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00576 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00725 ,  B01J2219/00743 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6825 ,  G01N21/6428 ,  G01N33/54373 ,  G01N2610/00 ,  Y10S436/805

    摘要： An assay element is described including recognition ligands bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of a biological target is described including injecting a biological target-containing sample into a sensor cell including the assay element, with the recognition ligands adapted for binding to selected biological targets, maintaining the sample within the sensor cell for time sufficient for binding to occur between selected biological targets within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting the fluorescent-label in any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

    摘要翻译： 描述了测定元件，其包括在单模平面光波导上与膜结合的识别配体，来自膜组的膜，聚合双层膜和其中含有聚乙二醇或聚丙二醇基团的自组装单层，以及 描述了用于检测生物靶的存在的测定方法，包括将包含生物靶标的样品注入包含测定元件的传感器单元中，其中识别配体适于结合选定的生物学靶标，将样品保持在传感器单元内以供时间 足以在样品内的选定生物靶和识别配体之间发生结合，将包含报道配体的溶液注入到传感器细胞中; 并且使用来自波导的激发光来询问传感器单元内的样本，由单一模式的消逝场提供的激发光穿透到含生物靶标的样品中，距离波导的距离小于约200纳米，由此令人兴奋 在距离波导小于约200纳米的距离内的任何结合的报道配体中的荧光标记，并产生可检测的信号。

    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers
    2.
    发明授权
    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers 有权
    基于平面光波导的夹心测定传感器和用于检测生物靶标的方法，包括癌症的早期检测

    公开(公告)号：US07541196B2

    公开(公告)日：2009-06-02

    申请号：US11172244

    申请日：2005-06-29

    申请人： Jennifer S. Martinez ,  Basil I. Swanson ,  John E. Shively ,  Lin Li

    发明人： Jennifer S. Martinez ,  Basil I. Swanson ,  John E. Shively ,  Lin Li

    IPC分类号： G01N33/551

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/57473 ,  G01N2610/00 ,  Y10S436/805

    摘要： An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of CEA is described including injecting a possible CEA-containing sample into a sensor cell including the assay element, maintaining the sample within the sensor cell for time sufficient for binding to occur between CEA present within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

    摘要翻译： 描述了一种测定元件，其包括适于结合到单模平面光波导上与膜结合的癌胚抗原（CEA）的识别配体，来自膜组的膜，聚合双层膜和含有 描述了其中的聚乙二醇或聚丙二醇基团，并且描述了用于检测CEA存在的测定方法，包括将可能的含CEA的样品注入到包含测定元件的传感器单元中，将样品保持在传感器单元内足以结合发生的时间 在样品中存在的CEA和识别配体之间，将包含报道分子配体的溶液注入到传感器单元中; 并且使用来自波导的激发光来询问传感器单元内的样本，由单一模式的消逝场提供的激发光穿透到含生物靶标的样品中，距离波导的距离小于约200纳米，由此令人兴奋 距离波导小于约200纳米的范围内的任何结合的报道配体，并产生可检测的信号。