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    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers
    1.
    发明授权
    Planar optical waveguide based sandwich assay sensors and processes for the detection of biological targets including early detection of cancers 有权
    基于平面光波导的夹心测定传感器和用于检测生物靶标的方法，包括癌症的早期检测

    公开(公告)号：US07541196B2

    公开(公告)日：2009-06-02

    申请号：US11172244

    申请日：2005-06-29

    申请人： Jennifer S. Martinez ,  Basil I. Swanson ,  John E. Shively ,  Lin Li

    发明人： Jennifer S. Martinez ,  Basil I. Swanson ,  John E. Shively ,  Lin Li

    IPC分类号： G01N33/551

    CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/57473 ,  G01N2610/00 ,  Y10S436/805

    摘要： An assay element is described including recognition ligands adapted for binding to carcinoembryonic antigen (CEA) bound to a film on a single mode planar optical waveguide, the film from the group of a membrane, a polymerized bilayer membrane, and a self-assembled monolayer containing polyethylene glycol or polypropylene glycol groups therein and an assay process for detecting the presence of CEA is described including injecting a possible CEA-containing sample into a sensor cell including the assay element, maintaining the sample within the sensor cell for time sufficient for binding to occur between CEA present within the sample and the recognition ligands, injecting a solution including a reporter ligand into the sensor cell; and, interrogating the sample within the sensor cell with excitation light from the waveguide, the excitation light provided by an evanescent field of the single mode penetrating into the biological target-containing sample to a distance of less than about 200 nanometers from the waveguide thereby exciting any bound reporter ligand within a distance of less than about 200 nanometers from the waveguide and resulting in a detectable signal.

    摘要翻译： 描述了一种测定元件，其包括适于结合到单模平面光波导上与膜结合的癌胚抗原（CEA）的识别配体，来自膜组的膜，聚合双层膜和含有 描述了其中的聚乙二醇或聚丙二醇基团，并且描述了用于检测CEA存在的测定方法，包括将可能的含CEA的样品注入到包含测定元件的传感器单元中，将样品保持在传感器单元内足以结合发生的时间 在样品中存在的CEA和识别配体之间，将包含报道分子配体的溶液注入到传感器单元中; 并且使用来自波导的激发光来询问传感器单元内的样本，由单一模式的消逝场提供的激发光穿透到含生物靶标的样品中，距离波导的距离小于约200纳米，由此令人兴奋 距离波导小于约200纳米的范围内的任何结合的报道配体，并产生可检测的信号。

    Process for conjugating biomolecules to hydrophobic membrane-incorporated molecules
    2.
    发明申请
    Process for conjugating biomolecules to hydrophobic membrane-incorporated molecules 审中-公开
    将生物分子与疏水性膜结合分子结合的方法

    公开(公告)号：US20110217459A1

    公开(公告)日：2011-09-08

    申请号：US11974970

    申请日：2007-10-16

    申请人： Basil I. Swanson ,  Clifford J. Unkefer ,  Jurgen G. Schmidt ,  Scott M. Reed ,  John E. Shively ,  Lin Li

    发明人： Basil I. Swanson ,  Clifford J. Unkefer ,  Jurgen G. Schmidt ,  Scott M. Reed ,  John E. Shively ,  Lin Li

    IPC分类号： B05D5/06

    CPC分类号： G01N33/5432

    摘要： A process is provided of conjugating a recognition element such as a biomolecule to a hydrophobic multifunctional linker molecule by incorporating a multifunctional linker molecule including one or more anchoring groups, a reporter group and a reactive site thereon into a membrane, and, reacting the membrane including the incorporated multifunctional linker molecule with a pre-selected recognition element to form a covalently bound recognition element-multifunctional linker molecule-membrane assembly. Also, a chemical assembly suitable for subsequent covalent attachment of a recognition element is provided such assembly including a multifunctional linker molecule including one or more anchoring groups, a reporter group, and a hydrophilic spacer terminated by a reactive group capable of subsequent covalent bonding, the one or more anchoring groups incorporated in a membrane.

    摘要翻译： 提供了通过将包括一个或多个锚定基团，报道基团和反应位点的多官能连接分子掺入到膜中，使识别元件例如生物分子与疏水多功能连接分子结合的方法， 所引入的多功能连接分子具有预选的识别元件以形成共价结合的识别元件 - 多功能连接子分子 - 膜组件。 此外，提供适于随后共价连接识别元件的化学组合物，其包括包含一个或多个锚定基团的多官能连接分子，报道基团和由能够随后共价键合的反应性基团封端的亲水性间隔基 一个或多个结合在膜中的锚定基团。

    Sequencing of protein immobilized on polytetrafluoroethylene supports
    3.
    发明授权
    Sequencing of protein immobilized on polytetrafluoroethylene supports 失效
    固定在聚四氟乙烯载体上的蛋白质测序

    公开(公告)号：US5541114A

    公开(公告)日：1996-07-30

    申请号：US204416

    申请日：1994-03-15

    申请人： Jerome M. Bailey ,  John E. Shively

    发明人： Jerome M. Bailey ,  John E. Shively

    IPC分类号： G01N33/545 ,  G01N33/68

    CPC分类号： G01N33/68 ,  G01N33/545

    摘要： A method for sequencing proteins on a polytetrafluoroethylene support is described. The support is preferably porous. The sample to be sequenced may be transferred directly, e.g., by blotting, to the support. Covalent binding of the sample to the support is not required.

    摘要翻译： PCT No.PCT / US92 / 06083 Sec。 371日期1994年3月15日 102（e）1994年3月15日PCT提交1992年7月23日PCT公布。 出版物WO94 / 02855 日期：1994年2月3日描述了聚四氟乙烯载体上的蛋白质测序方法。 载体优选是多孔的。 要测序的样品可以直接转印，例如通过印迹转移到载体上。 不需要样品与载体的共价结合。

    Generation of novel bone forming cells (monoosteophils) from LL-37 treated monocytes
    5.
    发明授权
    Generation of novel bone forming cells (monoosteophils) from LL-37 treated monocytes 有权

    公开(公告)号：US08513013B2

    公开(公告)日：2013-08-20

    申请号：US13249877

    申请日：2011-09-30

    申请人： Zhifang Zhang ,  John E. Shively

    发明人： Zhifang Zhang ,  John E. Shively

    IPC分类号： C12N5/02 ,  C12N5/077 ,  C12N5/0786

    CPC分类号： A61K39/00 ,  A61K35/32 ,  A61K38/48 ,  C12N5/0654 ,  C12N2501/155 ,  C12N2501/998 ,  C12N2506/115

    摘要： In one embodiment, a monocyte derived bone-forming cell population is provided. In one embodiment, the cell population comprises an isolated monocyte cell population treated with an effective dose of LL-37. In another embodiment, a method of producing a population of monocyte-derived bone-forming cells is provided. The method comprises obtaining a blood sample from a subject; isolating a population of monocytes from the blood sample; treating the isolated monocytes with an effective dose of LL-37; and culturing the LL-37 treated monocytes until they differentiate into the population of monocyte-derived bone-forming cells. In another embodiment, a method of treatment for a bone injury or bone disease is provided. The method comprises administering a therapeutically effective amount of a composition to a subject having the bone injury or disease, the composition comprising a population of monoosteophils.

    GENERATION OF NOVEL BONE FORMING CELLS (MONOOSTEOPHILS) FROM LL-37 TREATED MONOCYTES
    7.
    发明申请
    GENERATION OF NOVEL BONE FORMING CELLS (MONOOSTEOPHILS) FROM LL-37 TREATED MONOCYTES 有权
    从LL-37处理的单核细胞中产生新的骨形成细胞（单体细胞）

    公开(公告)号：US20120177689A1

    公开(公告)日：2012-07-12

    申请号：US13249877

    申请日：2011-09-30

    申请人： Zhifang Zhang ,  John E. Shively

    发明人： Zhifang Zhang ,  John E. Shively

    IPC分类号： A61K39/00 ,  C12N5/00 ,  A61P37/06 ,  A61P19/08 ,  A61P19/10 ,  A61K35/32 ,  C12N5/02

    CPC分类号： A61K39/00 ,  A61K35/32 ,  A61K38/48 ,  C12N5/0654 ,  C12N2501/155 ,  C12N2501/998 ,  C12N2506/115

    摘要： In one embodiment, a monocyte derived bone-forming cell population is provided. In one embodiment, the cell population comprises an isolated monocyte cell population treated with an effective dose of LL-37. In another embodiment, a method of producing a population of monocyte-derived bone-forming cells is provided. The method comprises obtaining a blood sample from a subject; isolating a population of monocytes from the blood sample; treating the isolated monocytes with an effective dose of LL-37; and culturing the LL-37 treated monocytes until they differentiate into the population of monocyte-derived bone-forming cells. In another embodiment, a method of treatment for a bone injury or bone disease is provided. The method comprises administering a therapeutically effective amount of a composition to a subject having the bone injury or disease, the composition comprising a population of monoosteophils.

    摘要翻译： 在一个实施方案中，提供了单核细胞衍生的骨形成细胞群体。 在一个实施方案中，细胞群体包含用有效剂量的LL-37处理的分离的单核细胞群体。 在另一个实施方案中，提供了一种产生单核细胞衍生的骨形成细胞群的方法。 该方法包括从受试者获得血样; 从血液样品中分离单核细胞群; 用有效剂量的LL-37处理分离的单核细胞; 并培养LL-37处理的单核细胞，直到它们分化成单核细胞衍生的骨形成细胞群体。 在另一个实施方案中，提供了治疗骨损伤或骨疾病的方法。 该方法包括向具有骨损伤或疾病的受试者施用治疗有效量的组合物，所述组合物包含单一嗜骨细胞群体。

    C-terminal sequencing of peptides which may include proline
    8.
    发明授权
    C-terminal sequencing of peptides which may include proline 失效
    肽的C末端测序可能包括脯氨酸

    公开(公告)号：US5998213A

    公开(公告)日：1999-12-07

    申请号：US532667

    申请日：1995-11-09

    申请人： Jerome M. Bailey ,  John E. Shively

    发明人： Jerome M. Bailey ,  John E. Shively

    IPC分类号： C07K1/12 ,  G01N33/68

    CPC分类号： C07K1/128

    摘要： A carboxy terminal protein sequencing process is disclosed which utilizes diphenyl phosphoroisothiocyanatidate and a heterocyclic amine to produce a thiohydantoin derivative of the C-terminal amino acid. The derivative is readily cleaved. The method is useful to sequence through all of the 20 naturally occuring amino acids.

    摘要翻译： PCT No.PCT / US94 / 01742 Sec。 371日期：1995年11月9日 102（e）日期1995年11月9日PCT 1994年2月15日PCT PCT。 公开号WO95 / 22060 日期1995年8月17日公开了羧基末端蛋白测序方法，其使用二苯基硫代硫氰酸二苯酯和杂环胺产生C-末端氨基酸的硫代乙内酰脲衍生物。 衍生物易于切割。 该方法可用于顺序通过所有20种天然存在的氨基酸。